Habitat loss and fragmentation have caused declines in swamp rabbit populations. In the northernmost regions of its range, the rabbit is particularly vulnerable during snowy days, as its dark coat makes it particularly visible against the white background, making it more vulnerable to predators. Snow cover constrains both hiding ability and availability of food resources.
Swamp rabbits maintain social order through displays of dominance, and communicate directly through vocalizations such as squeaks, chirps, squeals and alert calls. Higher-ranking males will display dominance over lower-ranking males more frequently, and will move within the home ranges of females more frequently, with the alpha male copulating most often in a given season. The system of male dominance prevents injury between subordinate breeding males under the alpha, as the lower-ranking individuals will avoid higher-ranking males to prevent conflict. When escaping, swamp rabbits will use confusing maneuvers to evade a pursuer, such as jumping onto a log, running to the end, then backtracking and jumping off the other side. They are excellent swimmers, and may also dive into water to escape, sometimes keeping only the nose and eyes above the water.
The swamp rabbit's breeding season varies across its range. It occurs year-round in Texas, but elsewhere may take place between February and August. Spermatogenesis occurs in October and November according to a Missouri study, and in Mississippi, recorded males had descended testes more often in December and February than in any other months. Estrus lasts one hour following induced ovulation. Gestation lasts 37 days on average. Up to three litters are produced annually, with each litter producing one to six young. Embryo resorption can occur in S. aquaticus in response to stress, causing loss of litters. This is attributed to flooding, which can cause overcrowding as well as directly inducing the release of stress hormones.
The swamp rabbit has an average lifespan of 1.8 years, but individuals living up to 9 years have been recorded. It is hunted in its native range, both for its products (meat and fur) and for sport, which has been noted as not significantly affecting populations. Agricultural development within its range has caused habitat loss, as these developments both alter the usable habitat and increase severity and frequency of flooding. The swamp rabbit is occasionally kept in captivity as a subject of study.
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