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Genetically modified animal
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<h2 id="production">Production</h2> <p class="note">Main article: <a href="/facts/Genetic_engineering_techniques/ahP1jNG1">Genetic engineering techniques</a></p><p>The process of genetically engineering mammals is a slow, tedious, and expensive process.<a class="footnote-ref" id="fnref:2" href="#fn:2"><sup>2</sup></a> As with other genetically modified organisms (GMOs), first genetic engineers must isolate the gene they wish to insert into the host organism. This can be taken from a <a href="/facts/Cell_(biology)/bLM9UQvy">cell</a> containing the gene<a class="footnote-ref" id="fnref:3" href="#fn:3"><sup>3</sup></a> or <a href="/facts/Gene_synthesis/ELoepoLA">artificially synthesised</a>.<a class="footnote-ref" id="fnref:4" href="#fn:4"><sup>4</sup></a> If the chosen gene or the donor organism's <a href="/facts/Genome/31Sm08JT">genome</a> has been well studied it may already be accessible from a <a href="/facts/Library_(biology)/0AJpQmBT">genetic library</a>. The <a href="/facts/Gene/e1swwPY6">gene</a> is then combined with other genetic elements, including a <a href="/facts/Promoter_(biology)/YDYBzLfg">promoter</a> and <a href="/facts/Terminator_(genetics)/mdR46gBa">terminator</a> region and usually a <a href="/facts/Selectable_marker/qVbDk4IQ">selectable marker</a>.<a class="footnote-ref" id="fnref:5" href="#fn:5"><sup>5</sup></a> </p><p>A number of techniques are available for <a href="/facts/Gene_delivery/RwfsLxFX">inserting the isolated gene into the host genome</a>. With animals <a href="/facts/DNA/nB89Iauz">DNA</a> is generally inserted into using <a href="/facts/Microinjection/NTqLhvVL">microinjection</a>, where it can be injected through the cell's <a href="/facts/Nuclear_envelope/IwRQ5BVL">nuclear envelope</a> directly into the <a href="/facts/Cell_nucleus/D7sFRa3c">nucleus</a>, or through the use of <a href="/facts/Viral_vectors/sqkIdcqo">viral vectors</a>.<a class="footnote-ref" id="fnref:6" href="#fn:6"><sup>6</sup></a> The first transgenic animals were produced by injecting viral DNA into embryos and then implanting the embryos in females.<a class="footnote-ref" id="fnref:7" href="#fn:7"><sup>7</sup></a> It is necessary to ensure that the inserted DNA is present in the <a href="/facts/Embryonic_stem_cells/P1n1eS1p">embryonic stem cells</a>.<a class="footnote-ref" id="fnref:8" href="#fn:8"><sup>8</sup></a> The embryo would develop and it would be hoped that some of the genetic material would be incorporated into the reproductive cells. Then researchers would have to wait until the animal reached breeding age and then offspring would be screened for presence of the gene in every cell, using <a href="/facts/Polymerase_chain_reaction/G0jWzJy1">PCR</a>, <a href="/facts/Southern_hybridization/HXrr0Gz3">Southern hybridization</a>, and <a href="/facts/DNA_sequencing/hsPsNCQW">DNA sequencing</a>.<a class="footnote-ref" id="fnref:9" href="#fn:9"><sup>9</sup></a> </p><p>New technologies are making genetic modifications easier and more precise.<a class="footnote-ref" id="fnref:10" href="#fn:10"><sup>10</sup></a> <a href="/facts/Gene_targeting/tax0nFmv">Gene targeting</a> techniques, which creates <a href="/facts/Double-strand_breaks/hsURuYYq">double-stranded breaks</a> and takes advantage on the <a href="/facts/Cell_(biology)/bLM9UQvy">cells</a> natural <a href="/facts/Homologous_recombination/WU0IIVVv">homologous recombination</a> repair systems, have been developed to target insertion to exact <a href="/facts/Locus_(genetics)/JcIT8wLm">locations</a>. <a href="/facts/Genome_editing/NX2XDX4P">Genome editing</a> uses artificially engineered <a href="/facts/Nuclease/79mnW28W">nucleases</a> that create breaks at specific points. There are four families of engineered nucleases: <a href="/facts/Meganuclease/1TGsTyQM">meganucleases</a>,<a class="footnote-ref" id="fnref:11" href="#fn:11"><sup>11</sup></a><a class="footnote-ref" id="fnref:12" href="#fn:12"><sup>12</sup></a> <a href="/facts/Zinc_finger_nuclease/f8LDUsT7">zinc finger nucleases</a>,<a class="footnote-ref" id="fnref:13" href="#fn:13"><sup>13</sup></a><a class="footnote-ref" id="fnref:14" href="#fn:14"><sup>14</sup></a> <a href="/facts/Transcription_activator-like_effector_nuclease/muonqIIx">transcription activator-like effector nucleases</a> (TALENs),<a class="footnote-ref" id="fnref:15" href="#fn:15"><sup>15</sup></a><a class="footnote-ref" id="fnref:16" href="#fn:16"><sup>16</sup></a> and the Cas9-guideRNA system (adapted from <a href="/facts/CRISPR/VqRDofqC">CRISPR</a>).<a class="footnote-ref" id="fnref:17" href="#fn:17"><sup>17</sup></a><a class="footnote-ref" id="fnref:18" href="#fn:18"><sup>18</sup></a> TALEN and CRISPR are the two most commonly used and each has its own advantages.<a class="footnote-ref" id="fnref:19" href="#fn:19"><sup>19</sup></a> TALENs have greater target specificity, while CRISPR is easier to design and more efficient.<a class="footnote-ref" id="fnref:20" href="#fn:20"><sup>20</sup></a> The development of the <a href="/facts/CRISPR/VqRDofqC">CRISPR-Cas9 gene editing</a> system has effectively halved the amount of time needed to develop genetically modified animals.<a class="footnote-ref" id="fnref:21" href="#fn:21"><sup>21</sup></a> </p> <p class="note">Main article: <a href="/facts/History_of_genetic_engineering/0Pn9DvfK">History of genetic engineering</a></p> <p>Humans have <a href="/facts/Domestication/tWB5s52s">domesticated</a> animals since around 12,000 BCE, using <a href="/facts/Selective_breeding/CAkkM2UG">selective breeding</a> or artificial selection (as contrasted with <a href="/facts/Natural_selection/JXTte6en">natural selection</a>). The process of <a href="/facts/Selective_breeding/CAkkM2UG">selective breeding</a>, in which organisms with desired <a href="/facts/Phenotypic_trait/seYSUTul">traits</a> (and thus with the desired <a href="/facts/Genes/e1swwPY6">genes</a>) are used to breed the next generation and organisms lacking the trait are not bred, is a precursor to the modern concept of genetic modification<a class="footnote-ref" id="fnref:22" href="#fn:22"><sup>22</sup></a>: 1 Various advancements in <a href="/facts/Genetics/bu8wne0A">genetics</a> allowed humans to directly alter the <a href="/facts/DNA/nB89Iauz">DNA</a> and therefore genes of organisms. In 1972, <a href="/facts/Paul_Berg/rTBQSxt3">Paul Berg</a> created the first <a href="/facts/Recombinant_DNA/dT3PL7M6">recombinant DNA</a> molecule when he combined DNA from a <a href="/facts/Monkey_virus/5nuFIf4a">monkey virus</a> with that of the <a href="/facts/Lambda_phage/z17FFUSW">lambda virus</a>.<a class="footnote-ref" id="fnref:23" href="#fn:23"><sup>23</sup></a><a class="footnote-ref" id="fnref:24" href="#fn:24"><sup>24</sup></a> </p><p>In 1974, <a href="/facts/Rudolf_Jaenisch/l0GvdIjm">Rudolf Jaenisch</a> created a <a href="/facts/Transgenic_mouse/cGycqMQ2">transgenic mouse</a> by introducing foreign DNA into its <a href="/facts/Embryo/9QHnLVLN">embryo</a>, making it the world's first transgenic animal.<a class="footnote-ref" id="fnref:25" href="#fn:25"><sup>25</sup></a><a class="footnote-ref" id="fnref:26" href="#fn:26"><sup>26</sup></a> However it took another eight years before transgenic mice were developed that passed the <a href="/facts/Transgene/8DvIYSGt">transgene</a> to their offspring.<a class="footnote-ref" id="fnref:27" href="#fn:27"><sup>27</sup></a><a class="footnote-ref" id="fnref:28" href="#fn:28"><sup>28</sup></a> Genetically modified mice were created in 1984 that carried cloned <a href="/facts/Oncogenes/WLFu6Ma5">oncogenes</a>, predisposing them to developing cancer.<a class="footnote-ref" id="fnref:29" href="#fn:29"><sup>29</sup></a> Mice with genes <a href="/facts/Gene_knockout/RJTMDbUR">knocked out</a> (<a href="/facts/Knockout_mouse/Aw50cobf">knockout mouse</a>) were created in 1989. The first transgenic livestock were produced in 1985<a class="footnote-ref" id="fnref:30" href="#fn:30"><sup>30</sup></a> and the first animal to synthesise transgenic proteins in their milk were mice,<a class="footnote-ref" id="fnref:31" href="#fn:31"><sup>31</sup></a> engineered to produce human tissue <a href="/facts/Plasminogen/bfLCyyPt">plasminogen</a> activator in 1987.<a class="footnote-ref" id="fnref:32" href="#fn:32"><sup>32</sup></a> </p><p>The first genetically modified animal to be commercialised was the <a href="/facts/GloFish/cv3yMGP8">GloFish</a>, a <a href="/facts/Zebrafish/Nxg1F1mG">Zebra fish</a> with a <a href="/facts/Fluorescent_protein/WQv6UQ7T">fluorescent gene</a> added that allows it to glow in the dark under <a href="/facts/Ultraviolet_light/kncBUqn5">ultraviolet light</a>.<a class="footnote-ref" id="fnref:33" href="#fn:33"><sup>33</sup></a> It was released to the US market in 2003.<a class="footnote-ref" id="fnref:34" href="#fn:34"><sup>34</sup></a> The first genetically modified animal to be approved for food use was <a href="/facts/AquAdvantage_salmon/Ctv2XBzG">AquAdvantage salmon</a> in 2015.<a class="footnote-ref" id="fnref:35" href="#fn:35"><sup>35</sup></a> The salmon were transformed with a <a href="/facts/Growth_hormone/SLEJ1fws">growth hormone</a>-regulating gene from a <a href="/facts/Chinook_salmon/o6WaX5zn">Pacific Chinook salmon</a> and a promoter from an <a href="/facts/Ocean_pout/6IcJXqN6">ocean pout</a> enabling it to grow year-round instead of only during spring and summer.<a class="footnote-ref" id="fnref:36" href="#fn:36"><sup>36</sup></a> </p> <h2 id="mammals">Mammals</h2> <p class="note">Main article: <a href="/facts/Genetically_modified_mammal/GetPamIY">Genetically modified mammal</a></p> <p>GM mammals are created for research purposes, production of industrial or therapeutic products, agricultural uses or improving their health. There is also a market for creating genetically modified pets.<a class="footnote-ref" id="fnref:37" href="#fn:37"><sup>37</sup></a> </p> <h3>Medicine</h3> <p>Mammals are the best <a href="/facts/Model/Hj8KMwdD">models</a> for human disease, making genetic engineered ones vital to the discovery and development of cures and treatments for many serious diseases. Knocking out genes responsible for <a href="/facts/Human_genetic_disorder/hFqt2NLn">human genetic disorders</a> allows researchers to study the mechanism of the disease and to test possible cures. <a href="/facts/Genetically_modified_mouse/cGycqMQ2">Genetically modified mice</a> have been the most common mammals used in <a href="/facts/Biomedical_research/lSVBFxDJ">biomedical research</a>, as they are cheap and easy to manipulate. Examples include <a href="/facts/Humanized_mouse/mpUDbpcv">humanized mice</a> created by <a href="/facts/Xenotransplantation/tbKt8gB5">xenotransplantation</a> of human gene products, so as to be utilized as murine <a href="/facts/Human-animal_hybrid/EL7qxd93">human-animal hybrids</a> for gaining relevant insights in the <i><a href="/facts/In_vivo/kWcWyl6f">in vivo</a></i> context for understanding of human-specific physiology and pathologies.<a class="footnote-ref" id="fnref:38" href="#fn:38"><sup>38</sup></a> Pigs are also a good target, because they have a similar body size, anatomical features, <a href="/facts/Physiology/fh25y2pQ">physiology</a>, <a href="/facts/Pathophysiology/Tp3Dny0c">pathophysiological</a> response, and diet.<a class="footnote-ref" id="fnref:39" href="#fn:39"><sup>39</sup></a> Nonhuman primates are the most similar model organisms to humans, but there is less public acceptance toward using them as research animals.<a class="footnote-ref" id="fnref:40" href="#fn:40"><sup>40</sup></a> In 2009, scientists announced that they had successfully transferred a gene into a <a href="/facts/Primate/Hl8RNE2X">primate</a> species (<a href="/facts/Marmoset/dR1wnBIq">marmosets</a>) and produced a stable line of breeding transgenic primates for the first time.<a class="footnote-ref" id="fnref:41" href="#fn:41"><sup>41</sup></a><a class="footnote-ref" id="fnref:42" href="#fn:42"><sup>42</sup></a> Their first research target for these marmosets was <a href="/facts/Parkinson%2527s_disease/xpekKj6x">Parkinson's disease</a>, but they were also considering <a href="/facts/Amyotrophic_lateral_sclerosis/9fIXnNL4">amyotrophic lateral sclerosis</a> and <a href="/facts/Huntington%2527s_disease/2P6yCoeK">Huntington's disease</a>.<a class="footnote-ref" id="fnref:43" href="#fn:43"><sup>43</sup></a> </p> <p>Human proteins expressed in mammals are more likely to be similar to their natural counterparts than those expressed in plants or microorganisms. Stable expression has been accomplished in sheep, pigs, rats, and other animals. In 2009, the first human biological drug produced from such an animal, a <a href="/facts/Goat/BrsPJmdX">goat</a>, was approved. The drug, <a href="/facts/ATryn/W5aJkcIk">ATryn</a>, is an <a href="/facts/Anticoagulant/cARpjm0G">anticoagulant</a> which reduces the probability of <a href="/facts/Blood_clot/vH6FDIQG">blood clots</a> during <a href="/facts/Surgery/fG8tWf5Y">surgery</a> or <a href="/facts/Childbirth/KaWFJ4kG">childbirth</a> was extracted from the goat's milk.<a class="footnote-ref" id="fnref:44" href="#fn:44"><sup>44</sup></a> Human <a href="/facts/Alpha-1_antitrypsin/T76VX84f">alpha-1-antitrypsin</a> is another protein that is used in treating humans with this deficiency.<a class="footnote-ref" id="fnref:45" href="#fn:45"><sup>45</sup></a> Another area is in creating pigs with greater capacity for <a href="/facts/Organ_transplantation/1jNvVJn6">human organ transplants</a> (<a href="/facts/Xenotransplantation/tbKt8gB5">xenotransplantation</a>). Pigs have been genetically modified so that their organs can no longer carry retroviruses<a class="footnote-ref" id="fnref:46" href="#fn:46"><sup>46</sup></a> or have modifications to reduce the chance of rejection.<a class="footnote-ref" id="fnref:47" href="#fn:47"><sup>47</sup></a><a class="footnote-ref" id="fnref:48" href="#fn:48"><sup>48</sup></a> Pig lungs from genetically modified pigs are being considered for transplantation into humans.<a class="footnote-ref" id="fnref:49" href="#fn:49"><sup>49</sup></a><a class="footnote-ref" id="fnref:50" href="#fn:50"><sup>50</sup></a> There is even potential to create chimeric pigs that can carry human organs.<a class="footnote-ref" id="fnref:51" href="#fn:51"><sup>51</sup></a><a class="footnote-ref" id="fnref:52" href="#fn:52"><sup>52</sup></a> </p> <h3>Livestock</h3> <p>Livestock are modified with the intention of improving economically important traits such as growth-rate, quality of meat, milk composition, disease resistance and survival. Animals have been engineered to grow faster, be healthier<a class="footnote-ref" id="fnref:53" href="#fn:53"><sup>53</sup></a> and resist diseases.<a class="footnote-ref" id="fnref:54" href="#fn:54"><sup>54</sup></a> Modifications have also improved the wool production of sheep and udder health of cows.<a class="footnote-ref" id="fnref:55" href="#fn:55"><sup>55</sup></a> </p><p>Goats have been genetically engineered to produce milk with strong spiderweb-like silk proteins.<a class="footnote-ref" id="fnref:56" href="#fn:56"><sup>56</sup></a> The goat gene sequence has been modified, using fresh umbilical cords taken from kids, in order to code for the human enzyme <a href="/facts/Lysozyme/VQqTSbFS">lysozyme</a>. Researchers wanted to alter the milk produced by the goats, to contain lysozyme in order to fight off bacteria causing <a href="/facts/Diarrhea/6TBHaRdA">diarrhea</a> in humans.<a class="footnote-ref" id="fnref:57" href="#fn:57"><sup>57</sup></a> </p><p>Enviropig was a genetically enhanced line of <a href="/facts/Yorkshire_pig/SQBm8Y6o">Yorkshire pigs</a> in Canada created with the capability of digesting plant <a href="/facts/Phosphorus/27xCeIqs">phosphorus</a> more efficiently than conventional Yorkshire pigs.<a class="footnote-ref" id="fnref:58" href="#fn:58"><sup>58</sup></a><a class="footnote-ref" id="fnref:59" href="#fn:59"><sup>59</sup></a> The A <a href="/facts/Transgene/8DvIYSGt">transgene</a> construct consisting of a <a href="/facts/Promoter_(genetics)/YDYBzLfg">promoter</a> expressed in the <a href="/facts/Murinae/7ds8prdo">murine</a> <a href="/facts/Parotid_gland/H4tPjP4D">parotid gland</a> and the <i><a href="/facts/Escherichia_coli/nR4Gvl56">Escherichia coli</a></i> <a href="/facts/Phytase/GQHd3x4U">phytase</a> gene was introduced into the pig embryo by pronuclear <a href="/facts/Microinjection/NTqLhvVL">microinjection</a>.<a class="footnote-ref" id="fnref:60" href="#fn:60"><sup>60</sup></a> This caused the pigs to produce the enzyme <a href="/facts/Phytase/GQHd3x4U">phytase</a>, which breaks down the indigestible phosphorus, in their saliva.<a class="footnote-ref" id="fnref:61" href="#fn:61"><sup>61</sup></a><a class="footnote-ref" id="fnref:62" href="#fn:62"><sup>62</sup></a> As a result, they excrete 30 to 70% less phosphorus in manure depending upon the age and diet.<a class="footnote-ref" id="fnref:63" href="#fn:63"><sup>63</sup></a><a class="footnote-ref" id="fnref:64" href="#fn:64"><sup>64</sup></a> The lower concentrations of phosphorus in <a href="/facts/Surface_runoff/zPxoaA0D">surface runoff</a> reduces <a href="/facts/Algae/U1ftVKU3">algal</a> growth, because phosphorus is the <a href="/facts/Limiting_nutrient/IYufWHvx">limiting nutrient</a> for algae.<a class="footnote-ref" id="fnref:65" href="#fn:65"><sup>65</sup></a> Because algae consume large amounts of oxygen, excessive growth can result in dead zones for fish. Funding for the Enviropig program ended in April 2012,<a class="footnote-ref" id="fnref:66" href="#fn:66"><sup>66</sup></a> and as no new partners were found the pigs were killed.<a class="footnote-ref" id="fnref:67" href="#fn:67"><sup>67</sup></a> However, the genetic material will be stored at the Canadian Agricultural Genetics Repository Program. In 2006, a pig was engineered to produce <a href="/facts/Omega-3_fatty_acid/X72Ynqov">omega-3 fatty acids</a> through the expression of a <a href="/facts/Roundworm/yLMJ43Eu">roundworm</a> gene.<a class="footnote-ref" id="fnref:68" href="#fn:68"><sup>68</sup></a> </p> <p>In 1990, the world's first transgenic <a href="/facts/Bovinae/SWIVbaIQ">bovine</a>, Herman the Bull, was developed. Herman was genetically engineered by micro-injected embryonic cells with the human gene coding for <a href="/facts/Lactoferrin/UpV8FC7f">lactoferrin</a>. The <a href="/facts/Dutch_Parliament/ekUD6R8K">Dutch Parliament</a> changed the law in 1992 to allow Herman to reproduce. Eight calves were born in 1994 and all calves inherited the lactoferrin gene.<a class="footnote-ref" id="fnref:69" href="#fn:69"><sup>69</sup></a> With subsequent sirings, Herman fathered a total of 83 calves.<a class="footnote-ref" id="fnref:70" href="#fn:70"><sup>70</sup></a> Dutch law required Herman to be <a href="/facts/Animal_slaughter/wRQW3ASl">slaughtered</a> at the conclusion of the <a href="/facts/Experiment/esltM54f">experiment</a>. However the Dutch Agriculture Minister at the time, <a href="/facts/Jozias_van_Aartsen/dWF7H9UW">Jozias van Aartsen</a>, granted him a reprieve provided he did not have more offspring after public and scientists rallied to his defence.<a class="footnote-ref" id="fnref:71" href="#fn:71"><sup>71</sup></a> Together with <a href="/facts/Cloning/Vb6SM4c7">cloned</a> cows named Holly and Belle, he lived out his retirement at <a href="/facts/Naturalis/WU6mPHj9">Naturalis</a>, the National Museum of Natural History in Leiden.<a class="footnote-ref" id="fnref:72" href="#fn:72"><sup>72</sup></a> On 2 April 2004, Herman was <a href="/facts/Animal_euthanasia/EECxNk9C">euthanised</a> by <a href="/facts/Veterinarian/MbJS5PKw">veterinarians</a> from the <a href="/facts/University_of_Utrecht/SfvqmD0l">University of Utrecht</a> because he suffered from <a href="/facts/Osteoarthritis/Iy1wDA1B">osteoarthritis</a>.<a class="footnote-ref" id="fnref:73" href="#fn:73"><sup>73</sup></a><a class="footnote-ref" id="fnref:74" href="#fn:74"><sup>74</sup></a> At the time of his death Herman was one of the oldest bulls in the Netherlands.<a class="footnote-ref" id="fnref:75" href="#fn:75"><sup>75</sup></a> Herman's hide has been preserved and mounted by <a href="/facts/Taxidermist/DG36uy0f">taxidermists</a> and is permanently on display in Naturalis. They say that he represents the start of a new era in the way man deals with nature, an icon of scientific progress, and the subsequent public discussion of these issues.<a class="footnote-ref" id="fnref:76" href="#fn:76"><sup>76</sup></a> </p><p>In October 2017, Chinese scientists announced they used <a href="/facts/CRISPR_gene_editing/nhPsllUR">CRISPR gene editing</a> technology to create of a line of pigs with better body temperature regulation, resulting in about 24% less body fat than typical livestock.<a class="footnote-ref" id="fnref:77" href="#fn:77"><sup>77</sup></a> </p><p>Researchers have developed GM dairy cattle to grow without horns (sometimes referred to as "<a href="/facts/Poll_(livestock)/Vue4F92V">polled</a>") which can cause injuries to farmers and other animals. DNA was taken from the genome of <a href="/facts/Red_Angus/AfuSlck8">Red Angus</a> cattle, which is known to suppress horn growth, and inserted into cells taken from an elite <a href="/facts/Holstein_Friesian_cattle/DNQcpXmq">Holstein</a> bull called "Randy". Each of the progeny will be a clone of Randy, but without his horns, and their offspring should also be hornless.<a class="footnote-ref" id="fnref:78" href="#fn:78"><sup>78</sup></a> In 2011, Chinese scientists generated <a href="/facts/Dairy_cows/DqGHuSXU">dairy cows</a> genetically engineered with genes from human beings to produce milk that would be the same as human breast milk.<a class="footnote-ref" id="fnref:79" href="#fn:79"><sup>79</sup></a> This could potentially benefit mothers who cannot produce breast milk but want their children to have breast milk rather than formula.<a class="footnote-ref" id="fnref:80" href="#fn:80"><sup>80</sup></a><a class="footnote-ref" id="fnref:81" href="#fn:81"><sup>81</sup></a> The researchers claim these transgenic cows to be identical to regular cows.<a class="footnote-ref" id="fnref:82" href="#fn:82"><sup>82</sup></a> Two months later, scientists from <a href="/facts/Argentina/aFSA5bAk">Argentina</a> presented Rosita, a transgenic cow incorporating two human genes, to produce milk with similar properties as human breast milk.<a class="footnote-ref" id="fnref:83" href="#fn:83"><sup>83</sup></a> In 2012, researchers from New Zealand also developed a genetically engineered cow that produced allergy-free milk.<a class="footnote-ref" id="fnref:84" href="#fn:84"><sup>84</sup></a> </p><p>In 2016 Jayne Raper and a team announced the first trypanotolerant transgenic cow in the world. This team, spanning the <a href="/facts/International_Livestock_Research_Institute/xNyaLKp6">International Livestock Research Institute</a>, <a href="/facts/Scotland%2527s_Rural_College/37vKApbe">Scotland's Rural College</a>, the <a href="/facts/Roslin_Institute/YQV9X66v">Roslin Institute</a>'s Centre for Tropical Livestock Genetics and Health, and the <a href="/facts/City_University_of_New_York/tSvG2Sgp">City University of New York</a>, announced that a <a href="/facts/Kenyan_Boran/w65BcPX6">Kenyan Boran</a> bull had been born and had already successfully had two children. Tumaini - named for the <a href="/facts/Swahili_language/sLzhutLC">Swahili</a> word for "hope" - carries a <a href="/facts/Trypanolytic_factor/uXbhrjVt">trypanolytic factor</a> from a <a href="/facts/Baboon/qz7J3AoC">baboon</a> via <a href="/facts/CRISPR%2fCas9/gDhwlw7I">CRISPR/Cas9</a>.<a class="footnote-ref" id="fnref:85" href="#fn:85"><sup>85</sup></a><a class="footnote-ref" id="fnref:86" href="#fn:86"><sup>86</sup></a> </p> <h3>Research</h3> <p>Scientists have genetically engineered several organisms, including some mammals, to include <a href="/facts/Green_fluorescent_protein/6nWDMhSC">green fluorescent protein</a> (GFP), for research purposes.<a class="footnote-ref" id="fnref:87" href="#fn:87"><sup>87</sup></a> GFP and other similar reporting genes allow easy visualisation and localisation of the products of the genetic modification.<a class="footnote-ref" id="fnref:88" href="#fn:88"><sup>88</sup></a> Fluorescent pigs have been bred to study human organ transplants, regenerating ocular <a href="/facts/Photoreceptor_cell/Ap0DcW5N">photoreceptor cells</a>, and other topics.<a class="footnote-ref" id="fnref:89" href="#fn:89"><sup>89</sup></a> In 2011 <a href="/facts/Green_fluorescent_protein/6nWDMhSC">green-fluorescent</a> cats were created to find therapies for <a href="/facts/HIV%2fAIDS/RqLIEMuJ">HIV/AIDS</a> and other diseases<a class="footnote-ref" id="fnref:90" href="#fn:90"><sup>90</sup></a> as <a href="/facts/Feline_immunodeficiency_virus/dYKMBf1V">feline immunodeficiency virus</a> (FIV) is related to HIV.<a class="footnote-ref" id="fnref:91" href="#fn:91"><sup>91</sup></a> Researchers from the University of Wyoming have developed a way to incorporate spiders' silk-spinning genes into goats, allowing the researchers to harvest the silk protein from the goats' milk for a variety of applications.<a class="footnote-ref" id="fnref:92" href="#fn:92"><sup>92</sup></a> </p> <h3>Conservation</h3> <p>Genetic modification of the <a href="/facts/Myxoma_virus/5fP0QvoT">myxoma virus</a> has been proposed to conserve <a href="/facts/European_rabbit/fPkGSd2x">European wild rabbits</a> in the <a href="/facts/Iberian_Peninsula/ztFXI8zt">Iberian peninsula</a> and to help regulate them in Australia. To protect the Iberian species from viral diseases, the myxoma virus was genetically modified to immunize the rabbits, while in Australia the same myxoma virus was genetically modified to lower fertility in the Australian rabbit population.<a class="footnote-ref" id="fnref:93" href="#fn:93"><sup>93</sup></a> There have also been suggestions that genetic engineering could be used to bring animals <a href="/facts/De-extinction/vgHRND7m">back from extinction</a>. It involves changing the genome of a close living relative to resemble the extinct one and is currently being attempted with the <a href="/facts/Passenger_pigeon/R0bQfP3p">passenger pigeon</a>.<a class="footnote-ref" id="fnref:94" href="#fn:94"><sup>94</sup></a> Genes associated with the <a href="/facts/Woolly_mammoth/6TtmERSs">woolly mammoth</a> have been added to the genome of an <a href="/facts/African_elephant/vMA6IWh1">African Elephant</a>, although the lead researcher says he has no intention of using live elephants.<a class="footnote-ref" id="fnref:95" href="#fn:95"><sup>95</sup></a> </p> <h3>Humans</h3> <p><a href="/facts/Gene_therapy/CaxRLfKM">Gene therapy</a><a class="footnote-ref" id="fnref:96" href="#fn:96"><sup>96</sup></a> uses genetically modified viruses to deliver genes which can cure disease in humans. Although gene therapy is still relatively new, it has had some successes. It has been used to treat <a href="/facts/Genetic_disorder/hFqt2NLn">genetic disorders</a> such as <a href="/facts/Severe_combined_immunodeficiency/gNfMUSp9">severe combined immunodeficiency</a><a class="footnote-ref" id="fnref:97" href="#fn:97"><sup>97</sup></a> and <a href="/facts/Adeno_associated_virus_and_gene_therapy_of_the_human_retina/zhrPF9Kv">Leber's congenital amaurosis</a>.<a class="footnote-ref" id="fnref:98" href="#fn:98"><sup>98</sup></a> Treatments are also being developed for a range of other currently incurable diseases, such as <a href="/facts/Cystic_fibrosis/tlNK5BAH">cystic fibrosis</a>,<a class="footnote-ref" id="fnref:99" href="#fn:99"><sup>99</sup></a> <a href="/facts/Sickle_cell_anemia/93LT7ATq">sickle cell anemia</a>,<a class="footnote-ref" id="fnref:100" href="#fn:100"><sup>100</sup></a> <a href="/facts/Parkinson%2527s_disease/xpekKj6x">Parkinson's disease</a>,<a class="footnote-ref" id="fnref:101" href="#fn:101"><sup>101</sup></a><a class="footnote-ref" id="fnref:102" href="#fn:102"><sup>102</sup></a> <a href="/facts/Cancer/PVW6n1D0">cancer</a>,<a class="footnote-ref" id="fnref:103" href="#fn:103"><sup>103</sup></a><a class="footnote-ref" id="fnref:104" href="#fn:104"><sup>104</sup></a><a class="footnote-ref" id="fnref:105" href="#fn:105"><sup>105</sup></a> <a href="/facts/Diabetes/2xeHXbsI">diabetes</a>,<a class="footnote-ref" id="fnref:106" href="#fn:106"><sup>106</sup></a> <a href="/facts/Heart_disease/HD420nMv">heart disease</a>,<a class="footnote-ref" id="fnref:107" href="#fn:107"><sup>107</sup></a> and <a href="/facts/Muscular_dystrophy/uU5xD0h8">muscular dystrophy</a>.<a class="footnote-ref" id="fnref:108" href="#fn:108"><sup>108</sup></a> These treatments only affect <a href="/facts/Somatic_cell/Emn7gk9A">somatic cells</a>, which means that any changes would not be inheritable. <a href="/facts/Germline/QI4bKK8Q">Germline</a> gene therapy results in any change being inheritable, which has raised concerns within the scientific community.<a class="footnote-ref" id="fnref:109" href="#fn:109"><sup>109</sup></a><a class="footnote-ref" id="fnref:110" href="#fn:110"><sup>110</sup></a> In 2015, CRISPR was used to edit the DNA of non-viable <a href="/facts/Human_embryos/9QHnLVLN">human embryos</a>.<a class="footnote-ref" id="fnref:111" href="#fn:111"><sup>111</sup></a><a class="footnote-ref" id="fnref:112" href="#fn:112"><sup>112</sup></a> In November 2018, <a href="/facts/He_Jiankui/D0Pdg8yK">He Jiankui</a> announced that he had <a href="/facts/Genome_editing/NX2XDX4P">edited the genomes</a> of two human embryos, to attempt to disable the <i><a href="/facts/CCR5/Ya83LIg8">CCR5</a></i> gene, which codes for a receptor that <a href="/facts/HIV/ulY2rgzu">HIV</a> uses to enter cells. He said that twin girls- <a href="/facts/Lulu_and_Nana/P2l1AcW0">Lulu and Nana</a>, had been born a few weeks earlier, and that they carried functional copies of CCR5 along with disabled CCR5 (<a href="/facts/Mosaicism/AtyxgmDS">mosaicism</a>), and were still vulnerable to HIV. The work was widely condemned as unethical, dangerous, and premature.<a class="footnote-ref" id="fnref:113" href="#fn:113"><sup>113</sup></a> </p> <h2 id="fish">Fish</h2> <p class="note">Main article: <a href="/facts/Genetically_modified_fish/tBYorAcf">Genetically modified fish</a></p> <p>Genetically modified fish are used for scientific research, as pets, and as a food source. <a href="/facts/Aquaculture/MYdIMfbt">Aquaculture</a> is a growing industry, currently providing over half of the consumed fish worldwide.<a class="footnote-ref" id="fnref:114" href="#fn:114"><sup>114</sup></a> Through genetic engineering, it is possible to increase growth rates, reduce food intake, remove <a href="/facts/Allergen/bnM4cttq">allergenic</a> properties, increase cold tolerance, and provide disease resistance. </p> <h3>Detecting pollution</h3> <p>Fish can also be used to detect aquatic pollution or function as bioreactors.<a class="footnote-ref" id="fnref:115" href="#fn:115"><sup>115</sup></a> Several groups have been developing <a href="/facts/Zebrafish/Nxg1F1mG">zebrafish</a> to detect <a href="/facts/Pollution/JRagxumQ">pollution</a> by attaching fluorescent proteins to genes activated by the presence of pollutants. The fish will then glow and can be used as environmental sensors.<a class="footnote-ref" id="fnref:116" href="#fn:116"><sup>116</sup></a><a class="footnote-ref" id="fnref:117" href="#fn:117"><sup>117</sup></a> </p> <h3>Pets</h3> <p>The <a href="/facts/GloFish/cv3yMGP8">GloFish</a> is a brand of genetically modified fluorescent <a href="/facts/Zebrafish/Nxg1F1mG">zebrafish</a> with bright red, green, and orange fluorescent color. It was originally developed by one of the groups to detect pollution, but is now part of the ornamental fish trade, becoming the first genetically modified animal to become publicly available as a pet when it was introduced for sale in 2003.<a class="footnote-ref" id="fnref:118" href="#fn:118"><sup>118</sup></a> </p> <h3>Research</h3> <p>GM fish are widely used in basic research in genetics and development. Two species of fish- zebrafish and <a href="/facts/Oryzias_latipes/usqgNXME">medaka</a>, are most commonly modified, because they have optically clear <a href="/facts/Chorion_(egg)/FmLILmRQ">chorions</a> (membranes in the egg), rapidly develop, and the 1-cell embryo is easy to see and microinject with transgenic DNA.<a class="footnote-ref" id="fnref:119" href="#fn:119"><sup>119</sup></a> Zebrafish are model organisms for developmental processes, <a href="/facts/Regeneration_(biology)/DHkSrakp">regeneration</a>, genetics, behaviour, disease mechanisms, and toxicity testing.<a class="footnote-ref" id="fnref:120" href="#fn:120"><sup>120</sup></a> Their transparency allows researchers to observe developmental stages, intestinal functions, and tumour growth.<a class="footnote-ref" id="fnref:121" href="#fn:121"><sup>121</sup></a><a class="footnote-ref" id="fnref:122" href="#fn:122"><sup>122</sup></a> The generation of transgenic protocols (whole organism, cell or tissue specific, tagged with reporter genes) has increased the level of information gained by studying these fish.<a class="footnote-ref" id="fnref:123" href="#fn:123"><sup>123</sup></a> </p> <h3>Growth</h3> <p>GM fish have been developed with promoters driving an over-production of "all fish" <a href="/facts/Growth_hormone/SLEJ1fws">growth hormone</a> for use in the <a href="/facts/Aquaculture/MYdIMfbt">aquaculture</a> industry, to increase the speed of development and potentially reduce fishing pressure on wild stocks. This has resulted in dramatic growth enhancement in several species, including <a href="/facts/Salmon/7kUsoqVN">salmon</a>,<a class="footnote-ref" id="fnref:124" href="#fn:124"><sup>124</sup></a> <a href="/facts/Trout/ptMf8sS2">trout</a>,<a class="footnote-ref" id="fnref:125" href="#fn:125"><sup>125</sup></a> and <a href="/facts/Tilapia/AI1hJJRS">tilapia</a>.<a class="footnote-ref" id="fnref:126" href="#fn:126"><sup>126</sup></a> </p><p><a href="/facts/AquaBounty_Technologies/4aqTKXyS">AquaBounty Technologies</a> have produced a salmon that can mature in half the time as wild salmon.<a class="footnote-ref" id="fnref:127" href="#fn:127"><sup>127</sup></a> The fish is an Atlantic salmon with a <a href="/facts/Chinook_salmon/o6WaX5zn">Chinook salmon</a> (<i>Oncorhynchus tshawytscha</i>) gene inserted. This allows the fish to produce growth hormones all year round compared to the wild-type fish that produces the hormone for only part of the year.<a class="footnote-ref" id="fnref:128" href="#fn:128"><sup>128</sup></a> The fish also has a second gene inserted from the eel-like <a href="/facts/Ocean_pout/6IcJXqN6">ocean pout</a> that acts like an "on" switch for the hormone.<a class="footnote-ref" id="fnref:129" href="#fn:129"><sup>129</sup></a> Pout also have <a href="/facts/Antifreeze_protein/Kvg6csG1">antifreeze proteins</a> in their blood, which allow the GM salmon to survive near-freezing waters and continue their development.<a class="footnote-ref" id="fnref:130" href="#fn:130"><sup>130</sup></a> A wild-type salmon takes 24 to 30 months to reach market size (4–6 kg), whereas the producers of the GM salmon say that it requires only 18 months for the GM fish to reach that size.<a class="footnote-ref" id="fnref:131" href="#fn:131"><sup>131</sup></a><a class="footnote-ref" id="fnref:132" href="#fn:132"><sup>132</sup></a><a class="footnote-ref" id="fnref:133" href="#fn:133"><sup>133</sup></a> In November 2015, the FDA of the USA approved the <a href="/facts/AquAdvantage_salmon/Ctv2XBzG">AquAdvantage salmon</a> for commercial production, sale, and consumption,<a class="footnote-ref" id="fnref:134" href="#fn:134"><sup>134</sup></a> the first non-plant GMO food to be commercialized.<a class="footnote-ref" id="fnref:135" href="#fn:135"><sup>135</sup></a> </p><p><a href="/facts/AquaBounty_Technologies/4aqTKXyS">AquaBounty</a> says that to prevent the genetically modified fish from inadvertently breeding with wild salmon, all of the fish will be female and reproductively sterile,<a class="footnote-ref" id="fnref:136" href="#fn:136"><sup>136</sup></a> although a small percentage of the females may remain fertile.<a class="footnote-ref" id="fnref:137" href="#fn:137"><sup>137</sup></a> Some opponents of the GM salmon have dubbed it the "Frankenfish".<a class="footnote-ref" id="fnref:138" href="#fn:138"><sup>138</sup></a><a class="footnote-ref" id="fnref:139" href="#fn:139"><sup>139</sup></a> </p> <h2 id="insects">Insects</h2> <p class="note">See also: <a href="/facts/Genetically_modified_insect/AxP1zkg8">Genetically modified insect</a></p> <h3>Research</h3> <p>In biological research, transgenic fruit flies (<i><a href="/facts/Drosophila_melanogaster/KtnHVCcB">Drosophila melanogaster</a></i>) are <a href="/facts/Model_organism/vNv6q4yb">model organisms</a> used to study the effects of genetic changes on development.<a class="footnote-ref" id="fnref:140" href="#fn:140"><sup>140</sup></a> Fruit flies are often preferred over other animals due to their short life cycle and low maintenance requirements. It also has a relatively simple genome compared to many <a href="/facts/Vertebrates/tT54FSTT">vertebrates</a>, with typically only one copy of each gene, making phenotypic analysis easy.<a class="footnote-ref" id="fnref:141" href="#fn:141"><sup>141</sup></a> <i>Drosophila</i> have been used to study genetics and inheritance, embryonic development, learning, behavior, and aging.<a class="footnote-ref" id="fnref:142" href="#fn:142"><sup>142</sup></a> <a href="/facts/Transposable_element/WbVMhuWR">Transposons</a> (particularly P elements) are well developed in <i>Drosophila</i> and provided an early method to add transgenes to their genome, although this has been taken over by more modern gene-editing techniques.<a class="footnote-ref" id="fnref:143" href="#fn:143"><sup>143</sup></a> </p> <h3>Population control</h3> <p>Due to their significance to human health, scientists are looking at ways to control mosquitoes through genetic engineering. Malaria-resistant mosquitoes have been developed in the laboratory.<a class="footnote-ref" id="fnref:144" href="#fn:144"><sup>144</sup></a> by inserting a gene that reduces the development of the malaria parasite<a class="footnote-ref" id="fnref:145" href="#fn:145"><sup>145</sup></a> and then use <a href="/facts/Homing_endonuclease/Gs54QBPr">homing endonucleases</a> to rapidly spread that gene throughout the male population (known as a <a href="/facts/Gene_drive/wqFg8qFp">gene drive</a>).<a class="footnote-ref" id="fnref:146" href="#fn:146"><sup>146</sup></a> This has been taken further by swapping it for a lethal gene.<a class="footnote-ref" id="fnref:147" href="#fn:147"><sup>147</sup></a><a class="footnote-ref" id="fnref:148" href="#fn:148"><sup>148</sup></a> In trials the populations of <i><a href="/facts/Aedes_aegypti/Y143uAaE">Aedes aegypti</a></i> mosquitoes, the single most important carrier of dengue fever and Zika virus, were reduced by between 80% and by 90%.<a class="footnote-ref" id="fnref:149" href="#fn:149"><sup>149</sup></a><a class="footnote-ref" id="fnref:150" href="#fn:150"><sup>150</sup></a><a class="footnote-ref" id="fnref:151" href="#fn:151"><sup>151</sup></a> Another approach is to use the <a href="/facts/Sterile_insect_technique/JM8Fpcd0">sterile insect technique</a>, whereby males genetically engineered to be sterile out compete viable males, to reduce population numbers.<a class="footnote-ref" id="fnref:152" href="#fn:152"><sup>152</sup></a> </p><p>Other <a href="/facts/Insect/IgDNvnUL">insect</a> pests that make attractive targets are <a href="/facts/Moth/bPrkvkGh">moths</a>. <a href="/facts/Diamondback_moth/oz8vlFct">Diamondback moths</a> cause US$4 to $5 billion of damage a year worldwide.<a class="footnote-ref" id="fnref:153" href="#fn:153"><sup>153</sup></a> The approach is similar to the mosquitoes, where males transformed with a gene that prevents females from reaching maturity will be released.<a class="footnote-ref" id="fnref:154" href="#fn:154"><sup>154</sup></a> They underwent field trials in 2017.<a class="footnote-ref" id="fnref:155" href="#fn:155"><sup>155</sup></a> Genetically modified moths have previously been released in field trials.<a class="footnote-ref" id="fnref:156" href="#fn:156"><sup>156</sup></a> A strain of <a href="/facts/Pink_bollworm/k0hb8qEh">pink bollworm</a> that were sterilised with radiation were genetically engineered to express a <a href="/facts/Green_fluorescent_protein/6nWDMhSC">red fluorescent protein</a> making it easier for researchers to monitor them.<a class="footnote-ref" id="fnref:157" href="#fn:157"><sup>157</sup></a> </p> <h3>Industry</h3> <p>Silkworm, the larvae stage of <i><a href="/facts/Bombyx_mori/r4IJIUtk">Bombyx mori</a>,</i> is an economically important insect in <a href="/facts/Sericulture/tW1uTTtf">sericulture</a>. Scientists are developing strategies to enhance silk quality and quantity. There is also potential to use the silk producing machinery to make other valuable proteins.<a class="footnote-ref" id="fnref:158" href="#fn:158"><sup>158</sup></a> Proteins expressed by silkworms include; <a href="/facts/Human_serum_albumin/wI8vu5py">human serum albumin</a>, <a href="/facts/Collagen_alpha-1(IV)_chain/Am2U6bul">human collagen α-chain</a>, mouse <a href="/facts/Monoclonal_antibody/4rUBWB87">monoclonal antibody</a> and <a href="/facts/NGLY1/fg9MUa0F">N-glycanase</a>.<a class="footnote-ref" id="fnref:159" href="#fn:159"><sup>159</sup></a> Silkworms have been created that produce <a href="/facts/Spider_silk/e1BwKC9S">spider silk</a>, a stronger but extremely difficult to harvest silk,<a class="footnote-ref" id="fnref:160" href="#fn:160"><sup>160</sup></a> and even novel silks.<a class="footnote-ref" id="fnref:161" href="#fn:161"><sup>161</sup></a> </p> <h2 id="birds">Birds</h2> <p>Attempts to produce genetically modified birds began before 1980.<a class="footnote-ref" id="fnref:162" href="#fn:162"><sup>162</sup></a> Chickens have been genetically modified for a variety of purposes. This includes studying <a href="/facts/Embryo_development/1lFY4EoW">embryo development</a>,<a class="footnote-ref" id="fnref:163" href="#fn:163"><sup>163</sup></a> preventing the transmission of <a href="/facts/Bird-flu/IhcLBsFU">bird flu</a><a class="footnote-ref" id="fnref:164" href="#fn:164"><sup>164</sup></a> and providing evolutionary insights using <a href="/facts/Reverse_engineering/pmJCV7J7">reverse engineering</a> to recreate dinosaur-like phenotypes.<a class="footnote-ref" id="fnref:165" href="#fn:165"><sup>165</sup></a> A GM chicken that produces the drug <a href="/facts/Kanuma_(drug)/vYoP95Pw">Kanuma</a>, an enzyme that treats a rare condition, in its egg passed regulatory approval in 2015.<a class="footnote-ref" id="fnref:166" href="#fn:166"><sup>166</sup></a> </p> <h3>Disease control</h3> <p>One potential use of GM birds could be to reduce the spread of avian disease. Researchers at <a href="/facts/Roslin_Institute/YQV9X66v">Roslin Institute</a> have produced a strain of GM chickens (<i>Gallus gallus domesticus</i>) that does not transmit <a href="/facts/Avian_flu/IhcLBsFU">avian flu</a> to other birds; however, these birds are still susceptible to contracting it. The genetic modification is an <a href="/facts/RNA/HxPeNfNj">RNA</a> molecule that prevents the virus reproduction by mimicking the region of the flu virus genome that controls replication. It is referred to as a "decoy" because it diverts the flu virus enzyme, the <a href="/facts/Polymerase/hN2ECr7B">polymerase</a>, from functions that are required for virus replication.<a class="footnote-ref" id="fnref:167" href="#fn:167"><sup>167</sup></a> </p> <h3>Evolutionary insights</h3> <p>A team of geneticists led by <a href="/facts/University_of_Montana/wWR1GY7n">University of Montana</a> paleontologist <a href="/facts/Jack_Horner_(paleontologist)/YfVGy8Be">Jack Horner</a> is seeking to modify a chicken to express several features present in ancestral <a href="/facts/Maniraptora/X0NxnsBy">maniraptorans</a> but absent in modern birds, such as teeth and a long tail,<a class="footnote-ref" id="fnref:168" href="#fn:168"><sup>168</sup></a> creating what has been dubbed a 'chickenosaurus'.<a class="footnote-ref" id="fnref:169" href="#fn:169"><sup>169</sup></a> Parallel projects have produced chicken embryos expressing dinosaur-like skull,<a class="footnote-ref" id="fnref:170" href="#fn:170"><sup>170</sup></a> leg,<a class="footnote-ref" id="fnref:171" href="#fn:171"><sup>171</sup></a> and foot<a class="footnote-ref" id="fnref:172" href="#fn:172"><sup>172</sup></a> anatomy. </p> <h3>In-ovo sexing</h3> <p class="note">Main article: <a href="/facts/In-ovo_sexing/XLvxGNJK">In-ovo sexing</a></p> <p>Gene editing is one possible tool in the laying hen breeding industry to provide an alternative to <a href="/facts/Chick_culling/Q3s7nCj5">Chick culling</a>. With this technology, breeding hens are given a genetic marker that is only passed down to male offspring. These males can then be identified during incubation and removed from the egg supply, so that only females hatch. For example, the Israeli startup eggXYt uses <a href="/facts/CRISPR/VqRDofqC">CRISPR</a> to give male eggs a biomarker that makes then glow under certain conditions.<a class="footnote-ref" id="fnref:173" href="#fn:173"><sup>173</sup></a> Importantly, the resulting laying hen and the eggs it producers are not themselves genetically edited. The European Union's Director General for Health and Food Safety has confirmed that made in this way eggs can be marketed,<a class="footnote-ref" id="fnref:174" href="#fn:174"><sup>174</sup></a> although none are commercially available as of June 2023.<a class="footnote-ref" id="fnref:175" href="#fn:175"><sup>175</sup></a> </p> <h2 id="amphibians">Amphibians</h2> <p>The first experiments that successfully developed <a href="/facts/Transgene/8DvIYSGt">transgenic</a> amphibians into embryos began in the 1980s with <i>Xenopus laevis</i>.<a class="footnote-ref" id="fnref:176" href="#fn:176"><sup>176</sup></a> Later, germline transgenic axolotls in <a href="/facts/Ambystoma_mexicanum/2UDp9cvb">Ambystoma mexicanum</a> were produced in 2006 using a technique called I-SceI-mediated transgenesis which utilizes the I-SceI endonuclease <a href="/facts/Enzyme/DSI1Fh7y">enzyme</a> that can break DNA at specific sites and allow for foreign DNA to be inserted into the genome.<a class="footnote-ref" id="fnref:177" href="#fn:177"><sup>177</sup></a> Both <i>Xenopus laevis</i> and Ambystoma mexicanum are model organisms used to study <a href="/facts/Regeneration_(biology)/DHkSrakp">regeneration</a>. In addition, transgenic lines have been produced in other <a href="/facts/Salamander/5pKurkwV">salamanders</a> including the Japanese newt Pyrrhogaster and <a href="/facts/Iberian_ribbed_newt/7XAA1lkC">Pleurodeles watl</a>.<a class="footnote-ref" id="fnref:178" href="#fn:178"><sup>178</sup></a> Genetically modified frogs, in particular <i><a href="/facts/African_clawed_frog/khG1KPrd">Xenopus laevis</a></i> and <i><a href="/facts/Western_clawed_frog/uoS2ALeT">Xenopus tropicalis</a></i>, are used in <a href="/facts/Development_(biology)/ckqaQlL7">development biology</a>. GM frogs can also be used as pollution sensors, especially for <a href="/facts/Endocrine_disruptor/Yk2hfnTj">endocrine disrupting chemicals</a>.<a class="footnote-ref" id="fnref:179" href="#fn:179"><sup>179</sup></a> There are proposals to use genetic engineering to control <a href="/facts/Cane_toads_in_Australia/Fk89Fn9w">cane toads in Australia</a>.<a class="footnote-ref" id="fnref:180" href="#fn:180"><sup>180</sup></a><a class="footnote-ref" id="fnref:181" href="#fn:181"><sup>181</sup></a> Many lines of transgenic X. laevis are used to study immunology to address how bacteria and viruses cause infectious disease at the University of Rochester Medical Center's X. laevis Research Resource for Immunobiology (XLRRI).<a class="footnote-ref" id="fnref:182" href="#fn:182"><sup>182</sup></a> Amphibians can also be used to study and validate regenerative <a href="/facts/Signaling_pathways/1bs0Me6w">signaling pathways</a> such as the <a href="/facts/WNT_pathway/7wxpKrjy">Wnt pathway</a>.<a class="footnote-ref" id="fnref:183" href="#fn:183"><sup>183</sup></a><a class="footnote-ref" id="fnref:184" href="#fn:184"><sup>184</sup></a> The wound-healing abilities of amphibians have many practical applications and can potentially provide a foundation for scar-free repair in human plastic surgery, such as treating the skin of burn patients.<a class="footnote-ref" id="fnref:185" href="#fn:185"><sup>185</sup></a> </p><p>Amphibians like X. laevis are suitable for experimental <a href="/facts/Embryology/4mvpSkhC">embryology</a> because they have large embryos that can be easily manipulated and observed during development.<a class="footnote-ref" id="fnref:186" href="#fn:186"><sup>186</sup></a> In experiments with axolotls, mutants with white pigmented skin are often used because their semi-transparent skin provides an efficient visualization and tracking method for <a href="/facts/Green_fluorescent_protein/6nWDMhSC">fluorescently tagged proteins</a> like <a href="/facts/Green_fluorescent_protein/6nWDMhSC">GFP</a>.<a class="footnote-ref" id="fnref:187" href="#fn:187"><sup>187</sup></a> Amphibians are not always ideal when it comes to the resources required to produce genetically modified animals; along with the one to two-year generation time, <a href="/facts/African_clawed_frog/khG1KPrd"><i>Xenopus laevis</i></a> can be considered less than ideal for transgenic experiments because of its <a href="/facts/Tetraploid/L7xyKHdK">pseudotetraploid</a> genome.<a class="footnote-ref" id="fnref:188" href="#fn:188"><sup>188</sup></a> Due to the same genes appearing in the genome multiple times, the chance of <a href="/facts/Mutagenesis/kQtI5zT1">mutagenesis</a> experiments working is lower.<a class="footnote-ref" id="fnref:189" href="#fn:189"><sup>189</sup></a> Current methods of freezing and thawing axolotl sperm render them nonfunctional, meaning transgenic lines must be maintained in a facility and this can get quite costly.<a class="footnote-ref" id="fnref:190" href="#fn:190"><sup>190</sup></a><a class="footnote-ref" id="fnref:191" href="#fn:191"><sup>191</sup></a> Producing transgenic axolotls has many challenges due to their large genome size.<a class="footnote-ref" id="fnref:192" href="#fn:192"><sup>192</sup></a> Current methods of generating transgenic axolotls are limited to random integration of the transgene <a href="/facts/Gene_cassette/VeqfwJ62">cassette</a> into the genome, which can lead to uneven expression or silencing.<a class="footnote-ref" id="fnref:193" href="#fn:193"><sup>193</sup></a> Gene duplicates also complicate efforts to generate efficient <a href="/facts/Gene_knockout/RJTMDbUR">gene knockouts</a>.<a class="footnote-ref" id="fnref:194" href="#fn:194"><sup>194</sup></a> </p><p>Despite the costs, axolotls have unique regenerative abilities and ultimately provide useful information in understanding tissue regeneration because they can regenerate their limbs, spinal cord, skin, heart, lungs, and other organs.<a class="footnote-ref" id="fnref:195" href="#fn:195"><sup>195</sup></a><a class="footnote-ref" id="fnref:196" href="#fn:196"><sup>196</sup></a> Naturally occurring mutant axolotls like the white strain that are often used in research have a transcriptional mutation at the Edn3 gene locus.<a class="footnote-ref" id="fnref:197" href="#fn:197"><sup>197</sup></a> Unlike other model organisms, the first fluorescently labeled cells in axolotls were differentiated muscle cells instead of embryos. In these initial experiments in the early 2000s, scientists were able to visualize muscle cell regeneration in the axolotl tail using a microinjecting technique, but cells could not be traced for the entire course of regeneration due to too harsh conditions that caused early cell death in labeled cells.<a class="footnote-ref" id="fnref:198" href="#fn:198"><sup>198</sup></a><a class="footnote-ref" id="fnref:199" href="#fn:199"><sup>199</sup></a> Though the process of producing transgenic axolotls was a challenge, scientists were able to label cells for longer durations using a plasmid transfection technique, which involves injecting DNA into cells using an electrical pulse in a process called <a href="/facts/Electroporation/Dx7tt65o">electroporation</a>. <a href="/facts/Transfection/R94fLSQF">Transfecting</a> axolotl cells is thought to be more difficult because of the composition of the <a href="/facts/Extracellular_matrix/kaHC5Ez8">extracellular matrix</a> (ECM). This technique allows spinal cord cells to be labeled and is very important in studying limb regeneration in many other cells; it has been used to study the role of the immune system in regeneration. Using <a href="/facts/Gene_knockout/RJTMDbUR">gene knockout</a> approaches, scientists can target specific regions of DNA using techniques like <a href="/facts/CRISPR%2fCas9-mediated_genome_editing/VqRDofqC">CRISPR/Cas9</a> to understand the function of certain genes based on the absence of the gene of interest. For example, gene knockouts of the <a href="/facts/SOX2/uKRxbGml">Sox2</a> gene confirm this region's role in neural stem cell amplification in the axolotl. The technology to do more complex conditional gene knockouts, or conditional knockouts that give the scientist spatiotemporal control of the gene is not yet suitable for axolotls.<a class="footnote-ref" id="fnref:200" href="#fn:200"><sup>200</sup></a> However, research in this field continues to develop and is made easier by recent sequencing of the genome and resources created for scientists, including data portals that contain axolotl genome and transcriptome reference assemblies to identify <a href="/facts/Orthologs/mCIVStrA">orthologs</a>.<a class="footnote-ref" id="fnref:201" href="#fn:201"><sup>201</sup></a><a class="footnote-ref" id="fnref:202" href="#fn:202"><sup>202</sup></a> </p> <h2 id="nematodes">Nematodes</h2> <p>The <a href="/facts/Nematode/yLMJ43Eu">nematode</a> <i><a href="/facts/Caenorhabditis_elegans/XJVJzPn2">Caenorhabditis elegans</a></i> is one of the major model organisms for researching <a href="/facts/Molecular_biology/YgqU35ub">molecular biology</a>.<a class="footnote-ref" id="fnref:203" href="#fn:203"><sup>203</sup></a> <a href="/facts/RNA_interference/oJRWBXJH">RNA interference</a> (RNAi) was discovered in <i>C. elegans</i><a class="footnote-ref" id="fnref:204" href="#fn:204"><sup>204</sup></a> and could be induced by simply feeding them bacteria modified to express <a href="/facts/Double-stranded_RNA/HxPeNfNj">double stranded RNA</a>.<a class="footnote-ref" id="fnref:205" href="#fn:205"><sup>205</sup></a> It is also relatively easy to produce stable transgenic nematodes and this along with RNAi are the major tools used in studying their genes.<a class="footnote-ref" id="fnref:206" href="#fn:206"><sup>206</sup></a> The most common use of transgenic nematodes has been studying gene expression and localisation by attaching reporter genes. Transgenes can also be combined with RNAi to rescue phenotypes, altered to study gene function, imaged in real time as the cells develop or used to control expression for different tissues or developmental stages.<a class="footnote-ref" id="fnref:207" href="#fn:207"><sup>207</sup></a> Transgenic nematodes have been used to study viruses,<a class="footnote-ref" id="fnref:208" href="#fn:208"><sup>208</sup></a> toxicology,<a class="footnote-ref" id="fnref:209" href="#fn:209"><sup>209</sup></a> and diseases<a class="footnote-ref" id="fnref:210" href="#fn:210"><sup>210</sup></a><a class="footnote-ref" id="fnref:211" href="#fn:211"><sup>211</sup></a> and to detect environmental pollutants.<a class="footnote-ref" id="fnref:212" href="#fn:212"><sup>212</sup></a> </p> <h2 id="other">Other</h2> <p>Systems have been developed to create transgenic organisms in a wide variety of other animals. The gene responsible for <a href="/facts/Albinism/o2FAg1UN">albinism</a> in <a href="/facts/Sea_cucumber/1ruJ7ePx">sea cucumbers</a> has been found, and used to engineer <a href="/facts/White_sea_cucumber/MSf61GPQ">white sea cucumbers</a>, a rare delicacy. The technology also opens the way to investigate the genes responsible for some of the cucumbers more unusual traits, including <a href="/facts/Hibernation/oltroTtp">hibernating</a> in summer, <a href="/facts/Evisceration_(autotomy)/b4gV9jaX">eviscerating</a> their intestines, and dissolving their bodies upon death.<a class="footnote-ref" id="fnref:213" href="#fn:213"><sup>213</sup></a> <a href="/facts/Flatworm/lvqbrb2V">Flatworms</a> have the ability to regenerate themselves from a single cell.<a class="footnote-ref" id="fnref:214" href="#fn:214"><sup>214</sup></a><a class="footnote-ref" id="fnref:215" href="#fn:215"><sup>215</sup></a> Until 2017 there was no effective way to transform them, which hampered research. By using microinjection and radiation, scientists have now created the first genetically modified flatworms.<a class="footnote-ref" id="fnref:216" href="#fn:216"><sup>216</sup></a> The <a href="/facts/Bristle_worm/Uo2YIINM">bristle worm</a>, a marine <a href="/facts/Annelid/PQvb7NAM">annelid</a>, has been modified. It is of interest due to its reproductive cycle being synchronized with lunar phases, regeneration capacity and slow evolution rate.<a class="footnote-ref" id="fnref:217" href="#fn:217"><sup>217</sup></a> <a href="/facts/Cnidarians/d0HRc6w0">Cnidaria</a> such as <i><a href="/facts/Hydra_(genus)/sdpMVp2e">Hydra</a></i> and the sea anemone <i><a href="/facts/Starlet_sea_anemone/QNVuhNU6">Nematostella vectensis</a></i> are attractive model organisms to study the <a href="/facts/Evolution/Hj0qAaBk">evolution</a> of <a href="/facts/Immunity_(medical)/vLPpzSNL">immunity</a> and certain developmental processes.<a class="footnote-ref" id="fnref:218" href="#fn:218"><sup>218</sup></a> Other organisms that have been genetically modified include <a href="/facts/Snail/y94YHrPw">snails</a>,<a class="footnote-ref" id="fnref:219" href="#fn:219"><sup>219</sup></a> <a href="/facts/Gecko/13Hu5c1w">geckos</a>, <a href="/facts/Turtle/X0ImnxYt">turtles</a>,<a class="footnote-ref" id="fnref:220" href="#fn:220"><sup>220</sup></a> <a href="/facts/Crayfish/aXYaXaV8">crayfish</a>, <a href="/facts/Oyster/ctvJgcks">oysters</a>, <a href="/facts/Shrimp/QeWUDe2b">shrimp</a>, <a href="/facts/Clam/5BB6vVl7">clams</a>, <a href="/facts/Abalone/qcq58R6J">abalone</a>,<a class="footnote-ref" id="fnref:221" href="#fn:221"><sup>221</sup></a> and <a href="/facts/Sponge/E9T2WFWA">sponges</a>.<a class="footnote-ref" id="fnref:222" href="#fn:222"><sup>222</sup></a> </p><p>Food products derived from genetically modified (GM) animals have not yet entered the European market. Nonetheless, the on-going discussion about GM crops [1], and the developing debate about the safety and ethics of foods and pharmaceutical products produced by both GM animals and plants, have provoked varying views across different sectors of society<a class="footnote-ref" id="fnref:223" href="#fn:223"><sup>223</sup></a> </p> <h2 id="ethics">Ethics</h2> <p><a href="/facts/Genetic_engineering/vwdlDR61">Genetic modification</a> and <a href="/facts/Genome_editing/NX2XDX4P">genome editing</a> hold potential for the future, but decisions regarding the use of these technologies must be based not only on what is possible, but also on what is ethically reasonable. Principles such as animal integrity, naturalness, risk identification and animal welfare are examples of ethically important factors that must be taken into consideration, and they also influence public perception and regulatory decisions by authorities.<a class="footnote-ref" id="fnref:224" href="#fn:224"><sup>224</sup></a> </p><p>The utility of extrapolating animal data to humans has been questioned. This has led ethical committees to adopt the principles of the four Rs (Reduction, Refinement, Replacement, and Responsibility) as a guide for decision-making regarding <a href="/facts/Animal_experimentation/mHMf98y8">animal experimentation</a>. However, complete abandonment of <a href="/facts/Laboratory_animals/CPXKNGUy">laboratory animals</a> has not yet been possible, and further research is needed to develop a roadmap for robust alternatives before their use can be fully discontinued.<a class="footnote-ref" id="fnref:225" href="#fn:225"><sup>225</sup></a> </p> <h2 id="references">References</h2> <ol> <li id="fn:1"><p>Forabosco F, Löhmus M, Rydhmer L, Sundström LF (May 2013). "Genetically modified farm animals and fish in agriculture: A review". Livestock Science. 153 (1–3): 1–9. doi:10.1016/j.livsci.2013.01.002. <a href="/wiki/Doi_(identifier)" target="_blank">/wiki/Doi_(identifier)</a> <a href="#fnref:1" class="footnote-back-ref">↩</a></p></li> <li id="fn:2"><p>Murray, Joo (20). Genetically modified animals Archived 2019-10-13 at the Wayback Machine. Canada: Brainwaving <a href="http://www.brainwaving.com/2010/07/28/genetically-modified-animals" target="_blank">http://www.brainwaving.com/2010/07/28/genetically-modified-animals</a> <a href="#fnref:2" class="footnote-back-ref">↩</a></p></li> <li id="fn:3"><p>Nicholl DS (2008-05-29). An Introduction to Genetic Engineering. Cambridge University Press. p. 34. ISBN 978-1-139-47178-7. <a href="978-1-139-47178-7" target="_blank">978-1-139-47178-7</a> <a href="#fnref:3" class="footnote-back-ref">↩</a></p></li> <li id="fn:4"><p>Liang J, Luo Y, Zhao H (2011). "Synthetic biology: putting synthesis into biology". Wiley Interdisciplinary Reviews: Systems Biology and Medicine. 3 (1): 7–20. doi:10.1002/wsbm.104. PMC 3057768. PMID 21064036. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3057768" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3057768</a> <a href="#fnref:4" class="footnote-back-ref">↩</a></p></li> <li id="fn:5"><p>Berg P, Mertz JE (January 2010). "Personal reflections on the origins and emergence of recombinant DNA technology". Genetics. 184 (1): 9–17. doi:10.1534/genetics.109.112144. PMC 2815933. PMID 20061565. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2815933" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2815933</a> <a href="#fnref:5" class="footnote-back-ref">↩</a></p></li> <li id="fn:6"><p>Chen I, Dubnau D (March 2004). "DNA uptake during bacterial transformation". Nature Reviews. Microbiology. 2 (3): 241–9. doi:10.1038/nrmicro844. PMID 15083159. S2CID 205499369. <a href="/wiki/Doi_(identifier)" target="_blank">/wiki/Doi_(identifier)</a> <a href="#fnref:6" class="footnote-back-ref">↩</a></p></li> <li id="fn:7"><p>Jaenisch R, Mintz B (April 1974). "Simian virus 40 DNA sequences in DNA of healthy adult mice derived from preimplantation blastocysts injected with viral DNA". Proceedings of the National Academy of Sciences of the United States of America. 71 (4): 1250–4. Bibcode:1974PNAS...71.1250J. doi:10.1073/pnas.71.4.1250. PMC 388203. PMID 4364530. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC388203" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC388203</a> <a href="#fnref:7" class="footnote-back-ref">↩</a></p></li> <li id="fn:8"><p>National Research Council (US) Committee on Identifying and Assessing Unintended Effects of Genetically Engineered Foods on Human Health (2004-01-01). Methods and Mechanisms for Genetic Manipulation of Plants, Animals, and Microorganisms. National Academies Press (US). <a href="https://www.ncbi.nlm.nih.gov/books/NBK215771/" target="_blank">https://www.ncbi.nlm.nih.gov/books/NBK215771/</a> <a href="#fnref:8" class="footnote-back-ref">↩</a></p></li> <li id="fn:9"><p>Setlow JK (2002-10-31). Genetic Engineering: Principles and Methods. Springer Science & Business Media. p. 109. ISBN 978-0-306-47280-0. <a href="978-0-306-47280-0" target="_blank">978-0-306-47280-0</a> <a href="#fnref:9" class="footnote-back-ref">↩</a></p></li> <li id="fn:10"><p>Murray, Joo (20). Genetically modified animals Archived 2019-10-13 at the Wayback Machine. Canada: Brainwaving <a href="http://www.brainwaving.com/2010/07/28/genetically-modified-animals" target="_blank">http://www.brainwaving.com/2010/07/28/genetically-modified-animals</a> <a href="#fnref:10" class="footnote-back-ref">↩</a></p></li> <li id="fn:11"><p>Grizot S, Smith J, Daboussi F, Prieto J, Redondo P, Merino N, et al. (September 2009). "Efficient targeting of a SCID gene by an engineered single-chain homing endonuclease". Nucleic Acids Research. 37 (16): 5405–19. doi:10.1093/nar/gkp548. PMC 2760784. PMID 19584299. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2760784" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2760784</a> <a href="#fnref:11" class="footnote-back-ref">↩</a></p></li> <li id="fn:12"><p>Gao H, Smith J, Yang M, Jones S, Djukanovic V, Nicholson MG, et al. (January 2010). "Heritable targeted mutagenesis in maize using a designed endonuclease". The Plant Journal. 61 (1): 176–87. doi:10.1111/j.1365-313X.2009.04041.x. PMID 19811621. <a href="https://doi.org/10.1111%2Fj.1365-313X.2009.04041.x" target="_blank">https://doi.org/10.1111%2Fj.1365-313X.2009.04041.x</a> <a href="#fnref:12" class="footnote-back-ref">↩</a></p></li> <li id="fn:13"><p>Townsend JA, Wright DA, Winfrey RJ, Fu F, Maeder ML, Joung JK, et al. (May 2009). "High-frequency modification of plant genes using engineered zinc-finger nucleases". Nature. 459 (7245): 442–5. Bibcode:2009Natur.459..442T. doi:10.1038/nature07845. PMC 2743854. PMID 19404258. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2743854" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2743854</a> <a href="#fnref:13" class="footnote-back-ref">↩</a></p></li> <li id="fn:14"><p>Shukla VK, Doyon Y, Miller JC, DeKelver RC, Moehle EA, Worden SE, et al. (May 2009). "Precise genome modification in the crop species Zea mays using zinc-finger nucleases". Nature. 459 (7245): 437–41. Bibcode:2009Natur.459..437S. doi:10.1038/nature07992. PMID 19404259. S2CID 4323298. <a href="/wiki/Bibcode_(identifier)" target="_blank">/wiki/Bibcode_(identifier)</a> <a href="#fnref:14" class="footnote-back-ref">↩</a></p></li> <li id="fn:15"><p>Christian M, Cermak T, Doyle EL, Schmidt C, Zhang F, Hummel A, et al. (October 2010). "Targeting DNA double-strand breaks with TAL effector nucleases". Genetics. 186 (2): 757–61. doi:10.1534/genetics.110.120717. PMC 2942870. PMID 20660643. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2942870" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2942870</a> <a href="#fnref:15" class="footnote-back-ref">↩</a></p></li> <li id="fn:16"><p>Li T, Huang S, Jiang WZ, Wright D, Spalding MH, Weeks DP, et al. (January 2011). "TAL nucleases (TALNs): hybrid proteins composed of TAL effectors and FokI DNA-cleavage domain". Nucleic Acids Research. 39 (1): 359–72. doi:10.1093/nar/gkq704. PMC 3017587. PMID 20699274. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3017587" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3017587</a> <a href="#fnref:16" class="footnote-back-ref">↩</a></p></li> <li id="fn:17"><p>Esvelt KM, Wang HH (2013). "Genome-scale engineering for systems and synthetic biology". Molecular Systems Biology. 9: 641. doi:10.1038/msb.2012.66. PMC 3564264. PMID 23340847. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3564264" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3564264</a> <a href="#fnref:17" class="footnote-back-ref">↩</a></p></li> <li id="fn:18"><p>Tan WS, Carlson DF, Walton MW, Fahrenkrug SC, Hackett PB (2012). "Precision editing of large animal genomes". Advances in Genetics Volume 80. Vol. 80. pp. 37–97. doi:10.1016/B978-0-12-404742-6.00002-8. ISBN 978-0-12-404742-6. PMC 3683964. PMID 23084873. <a href="978-0-12-404742-6" target="_blank">978-0-12-404742-6</a> <a href="#fnref:18" class="footnote-back-ref">↩</a></p></li> <li id="fn:19"><p>Malzahn A, Lowder L, Qi Y (2017-04-24). "Plant genome editing with TALEN and CRISPR". Cell & Bioscience. 7: 21. doi:10.1186/s13578-017-0148-4. PMC 5404292. PMID 28451378. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5404292" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5404292</a> <a href="#fnref:19" class="footnote-back-ref">↩</a></p></li> <li id="fn:20"><p>Malzahn A, Lowder L, Qi Y (2017-04-24). "Plant genome editing with TALEN and CRISPR". Cell & Bioscience. 7: 21. doi:10.1186/s13578-017-0148-4. PMC 5404292. PMID 28451378. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5404292" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5404292</a> <a href="#fnref:20" class="footnote-back-ref">↩</a></p></li> <li id="fn:21"><p>"How CRISPR is Spreading Through the Animal Kingdom". www.pbs.org. 23 May 2018. Retrieved 2018-12-20. <a href="https://www.pbs.org/wgbh/nova/article/crispr-animals/" target="_blank">https://www.pbs.org/wgbh/nova/article/crispr-animals/</a> <a href="#fnref:21" class="footnote-back-ref">↩</a></p></li> <li id="fn:22"><p>Clive Root (2007). Domestication. Greenwood Publishing Groups. <a href="https://books.google.com/books?id=WGDYHvOHwmwC" target="_blank">https://books.google.com/books?id=WGDYHvOHwmwC</a> <a href="#fnref:22" class="footnote-back-ref">↩</a></p></li> <li id="fn:23"><p>Jackson DA, Symons RH, Berg P (October 1972). "Biochemical method for inserting new genetic information into DNA of Simian Virus 40: circular SV40 DNA molecules containing lambda phage genes and the galactose operon of Escherichia coli". Proceedings of the National Academy of Sciences of the United States of America. 69 (10): 2904–9. Bibcode:1972PNAS...69.2904J. doi:10.1073/pnas.69.10.2904. PMC 389671. PMID 4342968. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC389671" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC389671</a> <a href="#fnref:23" class="footnote-back-ref">↩</a></p></li> <li id="fn:24"><p>M. K. Sateesh (25 August 2008). Bioethics And Biosafety. I. K. International Pvt Ltd. pp. 456–. ISBN 978-81-906757-0-3. Retrieved 27 March 2013. <a href="978-81-906757-0-3" target="_blank">978-81-906757-0-3</a> <a href="#fnref:24" class="footnote-back-ref">↩</a></p></li> <li id="fn:25"><p>Jaenisch, R. and Mintz, B. (1974 ) Simian virus 40 DNA sequences in DNA of healthy adult mice derived from preimplantation blastocysts injected with viral DNA. Proc. Natl. Acad. 71(4): 1250–54 [1] <a href="http://www.pnas.org/content/71/4/1250" target="_blank">http://www.pnas.org/content/71/4/1250</a> <a href="#fnref:25" class="footnote-back-ref">↩</a></p></li> <li id="fn:26"><p>"'Any idiot can do it.' Genome editor CRISPR could put mutant mice in everyone's reach". Science | AAAS. 2016-11-02. Retrieved 2016-12-02. <a href="https://www.science.org/content/article/any-idiot-can-do-it-genome-editor-crispr-could-put-mutant-mice-everyones-reach" target="_blank">https://www.science.org/content/article/any-idiot-can-do-it-genome-editor-crispr-could-put-mutant-mice-everyones-reach</a> <a href="#fnref:26" class="footnote-back-ref">↩</a></p></li> <li id="fn:27"><p>Gordon JW, Ruddle FH (December 1981). "Integration and stable germ line transmission of genes injected into mouse pronuclei". Science. 214 (4526): 1244–6. Bibcode:1981Sci...214.1244G. doi:10.1126/science.6272397. PMID 6272397. <a href="/wiki/Bibcode_(identifier)" target="_blank">/wiki/Bibcode_(identifier)</a> <a href="#fnref:27" class="footnote-back-ref">↩</a></p></li> <li id="fn:28"><p>Costantini F, Lacy E (November 1981). "Introduction of a rabbit beta-globin gene into the mouse germ line". Nature. 294 (5836): 92–4. Bibcode:1981Natur.294...92C. doi:10.1038/294092a0. PMID 6945481. S2CID 4371351. <a href="/wiki/Bibcode_(identifier)" target="_blank">/wiki/Bibcode_(identifier)</a> <a href="#fnref:28" class="footnote-back-ref">↩</a></p></li> <li id="fn:29"><p>Hanahan D, Wagner EF, Palmiter RD (September 2007). "The origins of oncomice: a history of the first transgenic mice genetically engineered to develop cancer". Genes & Development. 21 (18): 2258–70. doi:10.1101/gad.1583307. PMID 17875663. <a href="https://doi.org/10.1101%2Fgad.1583307" target="_blank">https://doi.org/10.1101%2Fgad.1583307</a> <a href="#fnref:29" class="footnote-back-ref">↩</a></p></li> <li id="fn:30"><p>Brophy B, Smolenski G, Wheeler T, Wells D, L'Huillier P, Laible G (February 2003). "Cloned transgenic cattle produce milk with higher levels of beta-casein and kappa-casein". Nature Biotechnology. 21 (2): 157–62. doi:10.1038/nbt783. PMID 12548290. S2CID 45925486. <a href="/wiki/Doi_(identifier)" target="_blank">/wiki/Doi_(identifier)</a> <a href="#fnref:30" class="footnote-back-ref">↩</a></p></li> <li id="fn:31"><p>Clark AJ (July 1998). "The mammary gland as a bioreactor: expression, processing, and production of recombinant proteins". Journal of Mammary Gland Biology and Neoplasia. 3 (3): 337–50. doi:10.1023/a:1018723712996. PMID 10819519. <a href="/wiki/Doi_(identifier)" target="_blank">/wiki/Doi_(identifier)</a> <a href="#fnref:31" class="footnote-back-ref">↩</a></p></li> <li id="fn:32"><p>Gordon K, Lee E, Vitale JA, Smith AE, Westphal H, Hennighausen L (1987). "Production of human tissue plasminogen activator in transgenic mouse milk. 1987". Biotechnology. 24 (11): 425–8. doi:10.1038/nbt1187-1183. PMID 1422049. S2CID 3261903. <a href="https://zenodo.org/record/1233349" target="_blank">https://zenodo.org/record/1233349</a> <a href="#fnref:32" class="footnote-back-ref">↩</a></p></li> <li id="fn:33"><p>Vàzquez-Salat N, Salter B, Smets G, Houdebine LM (2012-11-01). "The current state of GMO governance: are we ready for GM animals?". Biotechnology Advances. Special issue on ACB 2011. 30 (6): 1336–43. doi:10.1016/j.biotechadv.2012.02.006. PMID 22361646. <a href="/wiki/Doi_(identifier)" target="_blank">/wiki/Doi_(identifier)</a> <a href="#fnref:33" class="footnote-back-ref">↩</a></p></li> <li id="fn:34"><p>"CNN.com - Glowing fish to be first genetically changed pet - Nov. 21, 2003". CNN. Retrieved 2018-12-25. <a href="http://edition.cnn.com/2003/US/11/21/offbeat.glofish.reut/" target="_blank">http://edition.cnn.com/2003/US/11/21/offbeat.glofish.reut/</a> <a href="#fnref:34" class="footnote-back-ref">↩</a></p></li> <li id="fn:35"><p>"Aquabounty Cleared to Sell Salmon in USA for Commercial Purposes". FDA. 2019-06-19. <a href="https://www.fda.gov/AnimalVeterinary/DevelopmentApprovalProcess/GeneticEngineering/GeneticallyEngineeredAnimals/ucm280853.htm" target="_blank">https://www.fda.gov/AnimalVeterinary/DevelopmentApprovalProcess/GeneticEngineering/GeneticallyEngineeredAnimals/ucm280853.htm</a> <a href="#fnref:35" class="footnote-back-ref">↩</a></p></li> <li id="fn:36"><p>Bodnar A (October 2010). "Risk Assessment and Mitigation of AquAdvantage Salmon" (PDF). ISB News Report. Archived from the original (PDF) on 2021-03-08. Retrieved 2018-12-25. <a href="https://web.archive.org/web/20210308125138/https://aquabounty.com/wp-content/uploads/2014/02/Risk_Assessment_Mitigation_of_AAS-Oct2010.pdf" target="_blank">https://web.archive.org/web/20210308125138/https://aquabounty.com/wp-content/uploads/2014/02/Risk_Assessment_Mitigation_of_AAS-Oct2010.pdf</a> <a href="#fnref:36" class="footnote-back-ref">↩</a></p></li> <li id="fn:37"><p>Rudinko, Larisa (20). Guidance for industry. USA: Center for veterinary medicine Link. <a href="https://www.fda.gov/downloads/AnimalVeterinary/GuidanceComplianceEnforcement/GuidanceforIndustry/ucm113903.pdf/" target="_blank">https://www.fda.gov/downloads/AnimalVeterinary/GuidanceComplianceEnforcement/GuidanceforIndustry/ucm113903.pdf/</a> <a href="#fnref:37" class="footnote-back-ref">↩</a></p></li> <li id="fn:38"><p>Stripecke R, Münz C, Schuringa JJ, Bissig KD, Soper B, Meeham T, et al. (July 2020). "Innovations, challenges, and minimal information for standardization of humanized mice". EMBO Molecular Medicine. 12 (7): e8662. doi:10.15252/emmm.201708662. PMC 7338801. PMID 32578942. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7338801" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7338801</a> <a href="#fnref:38" class="footnote-back-ref">↩</a></p></li> <li id="fn:39"><p>Perleberg C, Kind A, Schnieke A (January 2018). "Genetically engineered pigs as models for human disease". Disease Models & Mechanisms. 11 (1): dmm030783. doi:10.1242/dmm.030783. PMC 5818075. PMID 29419487. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5818075" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5818075</a> <a href="#fnref:39" class="footnote-back-ref">↩</a></p></li> <li id="fn:40"><p>Sato K, Sasaki E (February 2018). "Genetic engineering in nonhuman primates for human disease modeling". Journal of Human Genetics. 63 (2): 125–131. doi:10.1038/s10038-017-0351-5. PMC 8075926. PMID 29203824. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8075926" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8075926</a> <a href="#fnref:40" class="footnote-back-ref">↩</a></p></li> <li id="fn:41"><p>Sasaki E, Suemizu H, Shimada A, Hanazawa K, Oiwa R, Kamioka M, et al. (May 2009). "Generation of transgenic non-human primates with germline transmission". Nature. 459 (7246): 523–7. Bibcode:2009Natur.459..523S. doi:10.1038/nature08090. PMID 19478777. S2CID 4404433. <a href="/wiki/Bibcode_(identifier)" target="_blank">/wiki/Bibcode_(identifier)</a> <a href="#fnref:41" class="footnote-back-ref">↩</a></p></li> <li id="fn:42"><p>Schatten G, Mitalipov S (May 2009). "Developmental biology: Transgenic primate offspring". Nature. 459 (7246): 515–6. Bibcode:2009Natur.459..515S. doi:10.1038/459515a. PMC 2777739. PMID 19478771. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2777739" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2777739</a> <a href="#fnref:42" class="footnote-back-ref">↩</a></p></li> <li id="fn:43"><p>Cyranoski D (May 2009). "Marmoset model takes centre stage". Nature. 459 (7246): 492. doi:10.1038/459492a. PMID 19478751. <a href="https://doi.org/10.1038%2F459492a" target="_blank">https://doi.org/10.1038%2F459492a</a> <a href="#fnref:43" class="footnote-back-ref">↩</a></p></li> <li id="fn:44"><p>Britt Erickson, 10 February 2009, for Chemical & Engineering News. FDA Approves Drug From Transgenic Goat Milk Accessed 6 October 2012 <a href="https://archive.today/20130112055323/http://pubs.acs.org/cen/news/87/i07/8707notw5.html" target="_blank">https://archive.today/20130112055323/http://pubs.acs.org/cen/news/87/i07/8707notw5.html</a> <a href="#fnref:44" class="footnote-back-ref">↩</a></p></li> <li id="fn:45"><p>Spencer LT, Humphries JE, Brantly ML (May 2005). "Antibody response to aerosolized transgenic human alpha1-antitrypsin". The New England Journal of Medicine. 352 (19): 2030–1. doi:10.1056/nejm200505123521923. PMID 15888711. <a href="https://doi.org/10.1056%2Fnejm200505123521923" target="_blank">https://doi.org/10.1056%2Fnejm200505123521923</a> <a href="#fnref:45" class="footnote-back-ref">↩</a></p></li> <li id="fn:46"><p>Zimmer C (15 October 2015). "Editing of Pig DNA May Lead to More Organs for People (Published 2015)". The New York Times. Archived from the original on 2022-12-16. <a href="https://www.nytimes.com/2015/10/20/science/editing-of-pig-dna-may-lead-to-more-organs-for-people.html?_r=1" target="_blank">https://www.nytimes.com/2015/10/20/science/editing-of-pig-dna-may-lead-to-more-organs-for-people.html?_r=1</a> <a href="#fnref:46" class="footnote-back-ref">↩</a></p></li> <li id="fn:47"><p>Zeyland J, Gawrońska B, Juzwa W, Jura J, Nowak A, Słomski R, et al. (August 2013). "Transgenic pigs designed to express human α-galactosidase to avoid humoral xenograft rejection". Journal of Applied Genetics. 54 (3): 293–303. doi:10.1007/s13353-013-0156-y. PMC 3720986. PMID 23780397. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3720986" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3720986</a> <a href="#fnref:47" class="footnote-back-ref">↩</a></p></li> <li id="fn:48"><p>GTKO study conducted by the National Heart, Lung, and Blood Institute of the U.S. National Institutes of Health <a href="https://www.iflscience.com/health-and-medicine/pig-heart-transplants-humans-could-be-their-way/" target="_blank">https://www.iflscience.com/health-and-medicine/pig-heart-transplants-humans-could-be-their-way/</a> <a href="#fnref:48" class="footnote-back-ref">↩</a></p></li> <li id="fn:49"><p>New life for pig-to-human transplants <a href="http://www.nature.com/news/new-life-for-pig-to-human-transplants-1.18768" target="_blank">http://www.nature.com/news/new-life-for-pig-to-human-transplants-1.18768</a> <a href="#fnref:49" class="footnote-back-ref">↩</a></p></li> <li id="fn:50"><p>United Therapeutics considering pig-lungs for transplant into humans <a href="https://geneticliteracyproject.org/2014/05/06/genetically-modified-pig-lungs-or-lab-grown-lungs-which-is-the-future-of-our-organ-supply/" target="_blank">https://geneticliteracyproject.org/2014/05/06/genetically-modified-pig-lungs-or-lab-grown-lungs-which-is-the-future-of-our-organ-supply/</a> <a href="#fnref:50" class="footnote-back-ref">↩</a></p></li> <li id="fn:51"><p>Perleberg C, Kind A, Schnieke A (January 2018). "Genetically engineered pigs as models for human disease". Disease Models & Mechanisms. 11 (1): dmm030783. doi:10.1242/dmm.030783. PMC 5818075. PMID 29419487. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5818075" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5818075</a> <a href="#fnref:51" class="footnote-back-ref">↩</a></p></li> <li id="fn:52"><p>Wu J, Platero-Luengo A, Sakurai M, Sugawara A, Gil MA, Yamauchi T, et al. (January 2017). "Interspecies Chimerism with Mammalian Pluripotent Stem Cells". Cell. 168 (3): 473–486.e15. doi:10.1016/j.cell.2016.12.036. PMC 5679265. PMID 28129541. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5679265" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5679265</a> <a href="#fnref:52" class="footnote-back-ref">↩</a></p></li> <li id="fn:53"><p>Lai L, Kang JX, Li R, Wang J, Witt WT, Yong HY, et al. (April 2006). "Generation of cloned transgenic pigs rich in omega-3 fatty acids". Nature Biotechnology. 24 (4): 435–6. doi:10.1038/nbt1198. PMC 2976610. PMID 16565727. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2976610" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2976610</a> <a href="#fnref:53" class="footnote-back-ref">↩</a></p></li> <li id="fn:54"><p>Tucker I (2018-06-24). "Genetically modified animals". The Guardian. ISSN 0261-3077. Retrieved 2018-12-21. <a href="https://www.theguardian.com/environment/2018/jun/24/genetically-engineered-animals-the-five-controversial-science" target="_blank">https://www.theguardian.com/environment/2018/jun/24/genetically-engineered-animals-the-five-controversial-science</a> <a href="#fnref:54" class="footnote-back-ref">↩</a></p></li> <li id="fn:55"><p>Forabosco F, Löhmus M, Rydhmer L, Sundström LF (May 2013). "Genetically modified farm animals and fish in agriculture: A review". Livestock Science. 153 (1–3): 1–9. doi:10.1016/j.livsci.2013.01.002. <a href="/wiki/Doi_(identifier)" target="_blank">/wiki/Doi_(identifier)</a> <a href="#fnref:55" class="footnote-back-ref">↩</a></p></li> <li id="fn:56"><p>Zyga L (2010). "Scientist bred goats that produce spider silk". Phys.org. Archived from the original on 30 April 2015. <a href="https://web.archive.org/web/20150430100830/http://phys.org/news194539934.html/" target="_blank">https://web.archive.org/web/20150430100830/http://phys.org/news194539934.html/</a> <a href="#fnref:56" class="footnote-back-ref">↩</a></p></li> <li id="fn:57"><p>"These GMO Goats Could Save Lives. Fear and Confusion Prevent It". Undark. Retrieved 2018-10-02. <a href="https://undark.org/article/gmo-goats-lysozyme-uc-davis-diarrhea/" target="_blank">https://undark.org/article/gmo-goats-lysozyme-uc-davis-diarrhea/</a> <a href="#fnref:57" class="footnote-back-ref">↩</a></p></li> <li id="fn:58"><p>Guelph (2010). Enviropig Archived 2016-01-30 at the Wayback Machine. Canada: <a href="http://www.uoguelph.ca/enviropig/index.shtml" target="_blank">http://www.uoguelph.ca/enviropig/index.shtml</a> <a href="#fnref:58" class="footnote-back-ref">↩</a></p></li> <li id="fn:59"><p>Schimdt, Sarah. "Genetically engineered pigs killed after funding ends", Postmedia News, 22 June 2012. Accessed 31 July 2012. <a href="http://www.canada.com/technology/science/Genetically+engineered+pigs+killed+after+funding+ends/6819844/story.html" target="_blank">http://www.canada.com/technology/science/Genetically+engineered+pigs+killed+after+funding+ends/6819844/story.html</a> <a href="#fnref:59" class="footnote-back-ref">↩</a></p></li> <li id="fn:60"><p>Golovan SP, Meidinger RG, Ajakaiye A, Cottrill M, Wiederkehr MZ, Barney DJ, et al. (August 2001). "Pigs expressing salivary phytase produce low-phosphorus manure". Nature Biotechnology. 19 (8): 741–5. doi:10.1038/90788. PMID 11479566. S2CID 52853680. <a href="/wiki/Doi_(identifier)" target="_blank">/wiki/Doi_(identifier)</a> <a href="#fnref:60" class="footnote-back-ref">↩</a></p></li> <li id="fn:61"><p>Guelph (2010). Enviropig Archived 2016-01-30 at the Wayback Machine. Canada: <a href="http://www.uoguelph.ca/enviropig/index.shtml" target="_blank">http://www.uoguelph.ca/enviropig/index.shtml</a> <a href="#fnref:61" class="footnote-back-ref">↩</a></p></li> <li id="fn:62"><p>Canada. "Enviropig – Environmental Benefits | University of Guelph". Uoguelph.ca. Archived from the original on 2017-10-30. <a href="https://web.archive.org/web/20171030215932/https://www.uoguelph.ca/enviropig/environmental_benefits.shtml" target="_blank">https://web.archive.org/web/20171030215932/https://www.uoguelph.ca/enviropig/environmental_benefits.shtml</a> <a href="#fnref:62" class="footnote-back-ref">↩</a></p></li> <li id="fn:63"><p>Guelph (2010). Enviropig Archived 2016-01-30 at the Wayback Machine. Canada: <a href="http://www.uoguelph.ca/enviropig/index.shtml" target="_blank">http://www.uoguelph.ca/enviropig/index.shtml</a> <a href="#fnref:63" class="footnote-back-ref">↩</a></p></li> <li id="fn:64"><p>Canada. "Enviropig – Environmental Benefits | University of Guelph". Uoguelph.ca. Archived from the original on 2017-10-30. <a href="https://web.archive.org/web/20171030215932/https://www.uoguelph.ca/enviropig/environmental_benefits.shtml" target="_blank">https://web.archive.org/web/20171030215932/https://www.uoguelph.ca/enviropig/environmental_benefits.shtml</a> <a href="#fnref:64" class="footnote-back-ref">↩</a></p></li> <li id="fn:65"><p>Guelph (2010). Enviropig Archived 2016-01-30 at the Wayback Machine. Canada: <a href="http://www.uoguelph.ca/enviropig/index.shtml" target="_blank">http://www.uoguelph.ca/enviropig/index.shtml</a> <a href="#fnref:65" class="footnote-back-ref">↩</a></p></li> <li id="fn:66"><p>Leung, Wendy. University of Guelph left foraging for Enviropig funding, The Globe and Mail, Apr. 2, 2012. Accessed July 31, 2012. <a href="https://www.theglobeandmail.com/news/national/university-of-guelph-left-foraging-for-enviropig-funding/article4097328/" target="_blank">https://www.theglobeandmail.com/news/national/university-of-guelph-left-foraging-for-enviropig-funding/article4097328/</a> <a href="#fnref:66" class="footnote-back-ref">↩</a></p></li> <li id="fn:67"><p>Schimdt, Sarah. Genetically engineered pigs killed after funding ends, Postmedia News, June 22, 2012. Accessed July 31, 2012. <a href="http://www.canada.com/technology/science/Genetically+engineered+pigs+killed+after+funding+ends/6819844/story.html" target="_blank">http://www.canada.com/technology/science/Genetically+engineered+pigs+killed+after+funding+ends/6819844/story.html</a> <a href="#fnref:67" class="footnote-back-ref">↩</a></p></li> <li id="fn:68"><p>Lai L, Kang JX, Li R, Wang J, Witt WT, Yong HY, et al. (April 2006). "Generation of cloned transgenic pigs rich in omega-3 fatty acids" (PDF). Nature Biotechnology. 24 (4): 435–6. doi:10.1038/nbt1198. PMC 2976610. PMID 16565727. Archived from the original (PDF) on 2009-08-16. <a href="https://web.archive.org/web/20090816010157/http://pmbcii.psy.cmu.edu/evans/2006_Lia.pdf" target="_blank">https://web.archive.org/web/20090816010157/http://pmbcii.psy.cmu.edu/evans/2006_Lia.pdf</a> <a href="#fnref:68" class="footnote-back-ref">↩</a></p></li> <li id="fn:69"><p>"Herman the bull - Herman becomes a father. "Biotech Notes."". U.S. Department of Agriculture. 1994. Archived from the original on 2008-12-03. <a href="https://web.archive.org/web/20081203150048/http://www.accessexcellence.org/RC/AB/IWT/Herman_the_Bull.php" target="_blank">https://web.archive.org/web/20081203150048/http://www.accessexcellence.org/RC/AB/IWT/Herman_the_Bull.php</a> <a href="#fnref:69" class="footnote-back-ref">↩</a></p></li> <li id="fn:70"><p>"Herman the bull heads to greener pastures". Expatica News. April 2, 2004. Archived from the original on July 29, 2014. Retrieved December 24, 2018. <a href="https://web.archive.org/web/20140729213117/http://www.expatica.com/nl/news/local_news/herman-the-bull-heads-to-greener-pastures--6273.html" target="_blank">https://web.archive.org/web/20140729213117/http://www.expatica.com/nl/news/local_news/herman-the-bull-heads-to-greener-pastures--6273.html</a> <a href="#fnref:70" class="footnote-back-ref">↩</a></p></li> <li id="fn:71"><p>"Herman the bull heads to greener pastures". Expatica News. April 2, 2004. Archived from the original on July 29, 2014. Retrieved December 24, 2018. <a href="https://web.archive.org/web/20140729213117/http://www.expatica.com/nl/news/local_news/herman-the-bull-heads-to-greener-pastures--6273.html" target="_blank">https://web.archive.org/web/20140729213117/http://www.expatica.com/nl/news/local_news/herman-the-bull-heads-to-greener-pastures--6273.html</a> <a href="#fnref:71" class="footnote-back-ref">↩</a></p></li> <li id="fn:72"><p>"Herman the bull heads to greener pastures". Expatica News. April 2, 2004. Archived from the original on July 29, 2014. Retrieved December 24, 2018. <a href="https://web.archive.org/web/20140729213117/http://www.expatica.com/nl/news/local_news/herman-the-bull-heads-to-greener-pastures--6273.html" target="_blank">https://web.archive.org/web/20140729213117/http://www.expatica.com/nl/news/local_news/herman-the-bull-heads-to-greener-pastures--6273.html</a> <a href="#fnref:72" class="footnote-back-ref">↩</a></p></li> <li id="fn:73"><p>"Herman the Bull stabled in Naturalis". Naturalis. 2008. Retrieved 3 January 2009.[dead link] <a href="http://www.naturalis.nl/naturalis.en/naturalis.en/i000968.html" target="_blank">http://www.naturalis.nl/naturalis.en/naturalis.en/i000968.html</a> <a href="#fnref:73" class="footnote-back-ref">↩</a></p></li> <li id="fn:74"><p>"Herman the bull heads to greener pastures". Expatica News. April 2, 2004. Archived from the original on July 29, 2014. Retrieved December 24, 2018. <a href="https://web.archive.org/web/20140729213117/http://www.expatica.com/nl/news/local_news/herman-the-bull-heads-to-greener-pastures--6273.html" target="_blank">https://web.archive.org/web/20140729213117/http://www.expatica.com/nl/news/local_news/herman-the-bull-heads-to-greener-pastures--6273.html</a> <a href="#fnref:74" class="footnote-back-ref">↩</a></p></li> <li id="fn:75"><p>"Herman the Bull stabled in Naturalis". Naturalis. 2008. Retrieved 3 January 2009.[dead link] <a href="http://www.naturalis.nl/naturalis.en/naturalis.en/i000968.html" target="_blank">http://www.naturalis.nl/naturalis.en/naturalis.en/i000968.html</a> <a href="#fnref:75" class="footnote-back-ref">↩</a></p></li> <li id="fn:76"><p>"Herman the Bull stabled in Naturalis". Naturalis. 2008. Retrieved 3 January 2009.[dead link] <a href="http://www.naturalis.nl/naturalis.en/naturalis.en/i000968.html" target="_blank">http://www.naturalis.nl/naturalis.en/naturalis.en/i000968.html</a> <a href="#fnref:76" class="footnote-back-ref">↩</a></p></li> <li id="fn:77"><p>"CRISPR Bacon: Chinese Scientists Create Genetically Modified Low-Fat Pigs". NPR.org. 2017-10-23. <a href="https://www.npr.org/sections/thesalt/2017/10/23/559060166/crispr-bacon-chinese-scientists-create-genetically-modified-low-fat-pigs" target="_blank">https://www.npr.org/sections/thesalt/2017/10/23/559060166/crispr-bacon-chinese-scientists-create-genetically-modified-low-fat-pigs</a> <a href="#fnref:77" class="footnote-back-ref">↩</a></p></li> <li id="fn:78"><p>Hall, M. (April 28, 2013). "Scientists design 'health and safety' cow with no horns". The Telegraph. Retrieved December 18, 2015. <a href="https://www.telegraph.co.uk/news/earth/agriculture/geneticmodification/10023561/Scientists-design-health-and-safety-cow-with-no-horns.html" target="_blank">https://www.telegraph.co.uk/news/earth/agriculture/geneticmodification/10023561/Scientists-design-health-and-safety-cow-with-no-horns.html</a> <a href="#fnref:78" class="footnote-back-ref">↩</a></p></li> <li id="fn:79"><p>Gray R (2011). "Genetically modified cows produce 'human' milk". The Telegraph. Archived from the original on April 4, 2011. <a href="https://web.archive.org/web/20110404101149/http://www.telegraph.co.uk/earth/agriculture/geneticmodification/8423536/Genetically-modified-cows-produce-human-milk.html" target="_blank">https://web.archive.org/web/20110404101149/http://www.telegraph.co.uk/earth/agriculture/geneticmodification/8423536/Genetically-modified-cows-produce-human-milk.html</a> <a href="#fnref:79" class="footnote-back-ref">↩</a></p></li> <li id="fn:80"><p>Classical Medicine Journal (14 April 2010). "Genetically modified cows producing human milk". Archived from the original on 6 November 2014. <a href="https://web.archive.org/web/20141106050820/http://www.classicalmedicinejournal.com/the-classical-medicine-journal/2011/4/13/genetically-modified-cows-producing-human-milk.html" target="_blank">https://web.archive.org/web/20141106050820/http://www.classicalmedicinejournal.com/the-classical-medicine-journal/2011/4/13/genetically-modified-cows-producing-human-milk.html</a> <a href="#fnref:80" class="footnote-back-ref">↩</a></p></li> <li id="fn:81"><p>Yapp R (11 June 2011). "Scientists create cow that produces 'human' milk". The Daily Telegraph. London. Retrieved 15 June 2012. <a href="https://www.telegraph.co.uk/news/worldnews/southamerica/argentina/8569687/Scientists-create-cow-that-produces-human-milk.html" target="_blank">https://www.telegraph.co.uk/news/worldnews/southamerica/argentina/8569687/Scientists-create-cow-that-produces-human-milk.html</a> <a href="#fnref:81" class="footnote-back-ref">↩</a></p></li> <li id="fn:82"><p>Classical Medicine Journal (14 April 2010). "Genetically modified cows producing human milk". Archived from the original on 2014-11-06. <a href="https://web.archive.org/web/20141106050820/http://www.classicalmedicinejournal.com/the-classical-medicine-journal/2011/4/13/genetically-modified-cows-producing-human-milk.html" target="_blank">https://web.archive.org/web/20141106050820/http://www.classicalmedicinejournal.com/the-classical-medicine-journal/2011/4/13/genetically-modified-cows-producing-human-milk.html</a> <a href="#fnref:82" class="footnote-back-ref">↩</a></p></li> <li id="fn:83"><p>Yapp R (11 June 2011). "Scientists create cow that produces 'human' milk". The Daily Telegraph. London. Retrieved 15 June 2012. <a href="https://www.telegraph.co.uk/news/worldnews/southamerica/argentina/8569687/Scientists-create-cow-that-produces-human-milk.html" target="_blank">https://www.telegraph.co.uk/news/worldnews/southamerica/argentina/8569687/Scientists-create-cow-that-produces-human-milk.html</a> <a href="#fnref:83" class="footnote-back-ref">↩</a></p></li> <li id="fn:84"><p>Jabed A, Wagner S, McCracken J, Wells DN, Laible G (October 2012). "Targeted microRNA expression in dairy cattle directs production of β-lactoglobulin-free, high-casein milk". Proceedings of the National Academy of Sciences of the United States of America. 109 (42): 16811–6. Bibcode:2012PNAS..10916811J. doi:10.1073/pnas.1210057109. PMC 3479461. PMID 23027958. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3479461" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3479461</a> <a href="#fnref:84" class="footnote-back-ref">↩</a></p></li> <li id="fn:85"><p>"Cloned bull could contribute to development of disease-resistant African cattle". ILRI news. 2016-09-05. Retrieved 2021-07-24. <a href="http://news.ilri.org/2016/09/05/cloned-bull-could-contribute-to-development-of-disease-resistant-african-cattle/" target="_blank">http://news.ilri.org/2016/09/05/cloned-bull-could-contribute-to-development-of-disease-resistant-african-cattle/</a> <a href="#fnref:85" class="footnote-back-ref">↩</a></p></li> <li id="fn:86"><p>Pal A, Chakravarty AK (22 October 2019). Genetics and breeding for disease resistance of livestock. London, United Kingdom: Academic Press. pp. 271–296. doi:10.1016/b978-0-12-816406-8.00019-x. ISBN 978-0-12-817267-4. OCLC 1125327298. PMC 7161387. S2CID 208596567. ISBN 978-0-12-816406-8 p. 276 <a href="978-0-12-817267-4" target="_blank">978-0-12-817267-4</a> <a href="#fnref:86" class="footnote-back-ref">↩</a></p></li> <li id="fn:87"><p>"Green fluorescent protein takes Nobel prize". Lewis Brindley. Retrieved 2015-05-31. <a href="http://www.rsc.org/chemistryworld/News/2008/October/08100802.asp" target="_blank">http://www.rsc.org/chemistryworld/News/2008/October/08100802.asp</a> <a href="#fnref:87" class="footnote-back-ref">↩</a></p></li> <li id="fn:88"><p>Alberts B, Johnson A, Lewis J, Raff M, Roberts K, Walter P (2002). "Studying Gene Expression and Function". Molecular Biology of the Cell (4th ed.). Garland Science. <a href="https://www.ncbi.nlm.nih.gov/books/NBK26818/" target="_blank">https://www.ncbi.nlm.nih.gov/books/NBK26818/</a> <a href="#fnref:88" class="footnote-back-ref">↩</a></p></li> <li id="fn:89"><p>Randall S (2008). e Harding S, p Tombs M (eds.). "Genetically Modified Pigs for Medicine and Agriculture" (PDF). Biotechnology and Genetic Engineering Reviews. 25: 245–66. doi:10.7313/upo9781904761679.011 (inactive 2024-11-02). ISBN 978-1-904761-67-9. PMID 21412358. Archived from the original (PDF) on 26 March 2014.{{cite journal}}: CS1 maint: DOI inactive as of November 2024 (link) <a href="978-1-904761-67-9" target="_blank">978-1-904761-67-9</a> <a href="#fnref:89" class="footnote-back-ref">↩</a></p></li> <li id="fn:90"><p>Wongsrikeao P, Saenz D, Rinkoski T, Otoi T, Poeschla E (September 2011). "Antiviral restriction factor transgenesis in the domestic cat". Nature Methods. 8 (10): 853–9. doi:10.1038/nmeth.1703. PMC 4006694. PMID 21909101. <a href="/wiki/Eric_Poeschla" target="_blank">/wiki/Eric_Poeschla</a> <a href="#fnref:90" class="footnote-back-ref">↩</a></p></li> <li id="fn:91"><p>Staff (3 April 2012). "Biology of HIV". National Institute of Allergy and Infectious Diseases. Archived from the original on 11 April 2014. <a href="https://web.archive.org/web/20140411035138/https://www.niaid.nih.gov/topics/hivaids/understanding/biology/Pages/biology.aspx" target="_blank">https://web.archive.org/web/20140411035138/https://www.niaid.nih.gov/topics/hivaids/understanding/biology/Pages/biology.aspx</a> <a href="#fnref:91" class="footnote-back-ref">↩</a></p></li> <li id="fn:92"><p>"Scientists breed goats that produce spider silk". Lisa Zyga , Phys.org. Retrieved May 31, 2010. <a href="https://phys.org/news/2010-05-scientists-goats-spider-silk.html" target="_blank">https://phys.org/news/2010-05-scientists-goats-spider-silk.html</a> <a href="#fnref:92" class="footnote-back-ref">↩</a></p></li> <li id="fn:93"><p>Angulo E, Cooke B (December 2002). "First synthesize new viruses then regulate their release? The case of the wild rabbit". Molecular Ecology. 11 (12): 2703–9. Bibcode:2002MolEc..11.2703A. doi:10.1046/j.1365-294X.2002.01635.x. hdl:10261/45541. PMID 12453252. S2CID 23916432. <a href="/wiki/Bibcode_(identifier)" target="_blank">/wiki/Bibcode_(identifier)</a> <a href="#fnref:93" class="footnote-back-ref">↩</a></p></li> <li id="fn:94"><p>Biello D. "Ancient DNA Could Return Passenger Pigeons to the Sky". Scientific American. Retrieved 2018-12-23. <a href="https://www.scientificamerican.com/article/ancient-dna-could-return-passenger-pigeons-to-the-sky/" target="_blank">https://www.scientificamerican.com/article/ancient-dna-could-return-passenger-pigeons-to-the-sky/</a> <a href="#fnref:94" class="footnote-back-ref">↩</a></p></li> <li id="fn:95"><p>Sarchet P. "Can we grow woolly mammoths in the lab? George Church hopes so". New Scientist. Press Association. Retrieved 2018-12-23. <a href="https://www.newscientist.com/article/2121503-can-we-grow-woolly-mammoths-in-the-lab-george-church-hopes-so/" target="_blank">https://www.newscientist.com/article/2121503-can-we-grow-woolly-mammoths-in-the-lab-george-church-hopes-so/</a> <a href="#fnref:95" class="footnote-back-ref">↩</a></p></li> <li id="fn:96"><p>Selkirk SM (October 2004). "Gene therapy in clinical medicine". Postgraduate Medical Journal. 80 (948): 560–70. doi:10.1136/pgmj.2003.017764. PMC 1743106. PMID 15466989. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1743106" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1743106</a> <a href="#fnref:96" class="footnote-back-ref">↩</a></p></li> <li id="fn:97"><p>Cavazzana-Calvo M, Fischer A (June 2007). "Gene therapy for severe combined immunodeficiency: are we there yet?". The Journal of Clinical Investigation. 117 (6): 1456–65. doi:10.1172/JCI30953. PMC 1878528. PMID 17549248. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1878528" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1878528</a> <a href="#fnref:97" class="footnote-back-ref">↩</a></p></li> <li id="fn:98"><p>Richards, Sabrina (6 November 2012) "Gene therapy arrives in Europe" The Scientist, Retrieved 15 April 2013 <a href="http://www.the-scientist.com/?articles.view/articleNo/33166/title/Gene-Therapy-Arrives-in-Europe/" target="_blank">http://www.the-scientist.com/?articles.view/articleNo/33166/title/Gene-Therapy-Arrives-in-Europe/</a> <a href="#fnref:98" class="footnote-back-ref">↩</a></p></li> <li id="fn:99"><p>Rosenecker J, Huth S, Rudolph C (October 2006). "Gene therapy for cystic fibrosis lung disease: current status and future perspectives". Current Opinion in Molecular Therapeutics. 8 (5): 439–45. PMID 17078386. <a href="/wiki/PMID_(identifier)" target="_blank">/wiki/PMID_(identifier)</a> <a href="#fnref:99" class="footnote-back-ref">↩</a></p></li> <li id="fn:100"><p>Persons DA, Nienhuis AW (July 2003). "Gene therapy for the hemoglobin disorders". Current Hematology Reports. 2 (4): 348–55. PMID 12901333. <a href="/wiki/PMID_(identifier)" target="_blank">/wiki/PMID_(identifier)</a> <a href="#fnref:100" class="footnote-back-ref">↩</a></p></li> <li id="fn:101"><p>LeWitt PA, Rezai AR, Leehey MA, Ojemann SG, Flaherty AW, Eskandar EN, et al. (April 2011). "AAV2-GAD gene therapy for advanced Parkinson's disease: a double-blind, sham-surgery controlled, randomised trial". The Lancet. Neurology. 10 (4): 309–19. doi:10.1016/S1474-4422(11)70039-4. PMID 21419704. S2CID 37154043. <a href="/wiki/Doi_(identifier)" target="_blank">/wiki/Doi_(identifier)</a> <a href="#fnref:101" class="footnote-back-ref">↩</a></p></li> <li id="fn:102"><p>Gallaher, James "Gene therapy 'treats' Parkinson's disease" BBC News Health, 17 March 2011. Retrieved 24 April 2011 <a href="https://www.bbc.co.uk/news/health-12758230" target="_blank">https://www.bbc.co.uk/news/health-12758230</a> <a href="#fnref:102" class="footnote-back-ref">↩</a></p></li> <li id="fn:103"><p>Urbina, Zachary (12 February 2013) "Genetically Engineered Virus Fights Liver Cancer Archived 16 February 2013 at the Wayback Machine" United Academics, Retrieved 15 February 2013 <a href="http://www.united-academics.org/magazine/health-medicine/killing-liver-cancer-with-a-genetically-engineered-virus/" target="_blank">http://www.united-academics.org/magazine/health-medicine/killing-liver-cancer-with-a-genetically-engineered-virus/</a> <a href="#fnref:103" class="footnote-back-ref">↩</a></p></li> <li id="fn:104"><p>"Treatment for Leukemia Is Showing Early Promise". The New York Times. Associated Press. 11 August 2011. p. A15. Retrieved 21 January 2013. <a href="https://www.nytimes.com/2011/08/11/health/research/11cancer.html" target="_blank">https://www.nytimes.com/2011/08/11/health/research/11cancer.html</a> <a href="#fnref:104" class="footnote-back-ref">↩</a></p></li> <li id="fn:105"><p>Coghlan, Andy (26 March 2013) "Gene therapy cures leukaemia in eight days" The New Scientist, Retrieved 15 April 2013 <a href="https://www.newscientist.com/article/mg21729104.100-gene-therapy-cures-leukaemia-in-eight-days.html" target="_blank">https://www.newscientist.com/article/mg21729104.100-gene-therapy-cures-leukaemia-in-eight-days.html</a> <a href="#fnref:105" class="footnote-back-ref">↩</a></p></li> <li id="fn:106"><p>Staff (13 February 2013) "Gene therapy cures diabetic dogs" New Scientist, Retrieved 15 February 2013 <a href="https://www.newscientist.com/article/mg21729044.800-gene-therapy-cures-diabetic-dogs.html" target="_blank">https://www.newscientist.com/article/mg21729044.800-gene-therapy-cures-diabetic-dogs.html</a> <a href="#fnref:106" class="footnote-back-ref">↩</a></p></li> <li id="fn:107"><p>(30 April 2013) "New gene therapy trial gives hope to people with heart failure" British Heart Foundation, Retrieved 5 May 2013 <a href="http://www.bhf.org.uk/default.aspx?page=16039" target="_blank">http://www.bhf.org.uk/default.aspx?page=16039</a> <a href="#fnref:107" class="footnote-back-ref">↩</a></p></li> <li id="fn:108"><p>Foster K, Foster H, Dickson JG (December 2006). "Gene therapy progress and prospects: Duchenne muscular dystrophy". Gene Therapy. 13 (24): 1677–85. doi:10.1038/sj.gt.3302877. PMID 17066097. <a href="https://doi.org/10.1038%2Fsj.gt.3302877" target="_blank">https://doi.org/10.1038%2Fsj.gt.3302877</a> <a href="#fnref:108" class="footnote-back-ref">↩</a></p></li> <li id="fn:109"><p>"1990 The Declaration of Inuyama". 5 August 2001. Archived from the original on 5 August 2001.{{cite web}}: CS1 maint: bot: original URL status unknown (link) <a href="https://web.archive.org/web/20010805085535/http://www.cioms.ch/frame_1990_texts_of_guidelines.htm" target="_blank">https://web.archive.org/web/20010805085535/http://www.cioms.ch/frame_1990_texts_of_guidelines.htm</a> <a href="#fnref:109" class="footnote-back-ref">↩</a></p></li> <li id="fn:110"><p>Smith KR, Chan S, Harris J (Oct 2012). "Human germline genetic modification: scientific and bioethical perspectives". Arch Med Res. 43 (7): 491–513. doi:10.1016/j.arcmed.2012.09.003. PMID 23072719. <a href="/wiki/Doi_(identifier)" target="_blank">/wiki/Doi_(identifier)</a> <a href="#fnref:110" class="footnote-back-ref">↩</a></p></li> <li id="fn:111"><p>Kolata G (23 April 2015). "Chinese Scientists Edit Genes of Human Embryos, Raising Concerns". The New York Times. Retrieved 24 April 2015. <a href="https://www.nytimes.com/2015/04/24/health/chinese-scientists-edit-genes-of-human-embryos-raising-concerns.html" target="_blank">https://www.nytimes.com/2015/04/24/health/chinese-scientists-edit-genes-of-human-embryos-raising-concerns.html</a> <a href="#fnref:111" class="footnote-back-ref">↩</a></p></li> <li id="fn:112"><p>Liang P, Xu Y, Zhang X, Ding C, Huang R, Zhang Z, et al. (May 2015). "CRISPR/Cas9-mediated gene editing in human tripronuclear zygotes". Protein & Cell. 6 (5): 363–372. doi:10.1007/s13238-015-0153-5. PMC 4417674. PMID 25894090. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4417674" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4417674</a> <a href="#fnref:112" class="footnote-back-ref">↩</a></p></li> <li id="fn:113"><p>Begley S (28 November 2018). "Amid uproar, Chinese scientist defends creating gene-edited babies – STAT". STAT. <a href="https://www.statnews.com/2018/11/28/chinese-scientist-defends-creating-gene-edited-babies/" target="_blank">https://www.statnews.com/2018/11/28/chinese-scientist-defends-creating-gene-edited-babies/</a> <a href="#fnref:113" class="footnote-back-ref">↩</a></p></li> <li id="fn:114"><p>"Half Of Fish Consumed Globally Is Now Raised On Farms, Study Finds". ScienceDaily. Retrieved 2018-12-21. <a href="https://www.sciencedaily.com/releases/2009/09/090907162320.htm" target="_blank">https://www.sciencedaily.com/releases/2009/09/090907162320.htm</a> <a href="#fnref:114" class="footnote-back-ref">↩</a></p></li> <li id="fn:115"><p>Tonelli FM, Lacerda SM, Tonelli FC, Costa GM, De França LR, Resende RR (2017-11-01). "Progress and biotechnological prospects in fish transgenesis". Biotechnology Advances. 35 (6): 832–844. doi:10.1016/j.biotechadv.2017.06.002. ISSN 0734-9750. PMID 28602961. <a href="/wiki/Doi_(identifier)" target="_blank">/wiki/Doi_(identifier)</a> <a href="#fnref:115" class="footnote-back-ref">↩</a></p></li> <li id="fn:116"><p>Nebert DW, Stuart GW, Solis WA, Carvan MJ (January 2002). "Use of reporter genes and vertebrate DNA motifs in transgenic zebrafish as sentinels for assessing aquatic pollution". Environmental Health Perspectives. 110 (1): A15. doi:10.1289/ehp.110-1240712. PMC 1240712. PMID 11813700. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1240712" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1240712</a> <a href="#fnref:116" class="footnote-back-ref">↩</a></p></li> <li id="fn:117"><p>Mattingly CJ, McLachlan JA, Toscano WA (August 2001). "Green fluorescent protein (GFP) as a marker of aryl hydrocarbon receptor (AhR) function in developing zebrafish (Danio rerio)". Environmental Health Perspectives. 109 (8): 845–9. Bibcode:2001EnvHP.109..845M. doi:10.1289/ehp.01109845. PMC 1240414. PMID 11564622. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1240414" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1240414</a> <a href="#fnref:117" class="footnote-back-ref">↩</a></p></li> <li id="fn:118"><p>Hallerman E (June 2004). "Glofish, the first GM animal commercialized: profits amid controversy". ISB News Report. <a href="http://www.isb.vt.edu/articles/jun0405.htm" target="_blank">http://www.isb.vt.edu/articles/jun0405.htm</a> <a href="#fnref:118" class="footnote-back-ref">↩</a></p></li> <li id="fn:119"><p>Hackett PB, Ekker SE, Essner JJ (2004). "Chapter 16: Applications of transposable elements in fish for transgenesis and functional genomics". In Gong Z, Korzh V (eds.). Fish Development and Genetics. World Scientific, Inc. pp. 532–80. <a href="#fnref:119" class="footnote-back-ref">↩</a></p></li> <li id="fn:120"><p>Meyers JR (2018). "Zebrafish: Development of a Vertebrate Model Organism". Current Protocols in Essential Laboratory Techniques. 16 (1): e19. doi:10.1002/cpet.19. <a href="https://doi.org/10.1002%2Fcpet.19" target="_blank">https://doi.org/10.1002%2Fcpet.19</a> <a href="#fnref:120" class="footnote-back-ref">↩</a></p></li> <li id="fn:121"><p>Lu JW, Ho YJ, Ciou SC, Gong Z (September 2017). "Innovative Disease Model: Zebrafish as an In Vivo Platform for Intestinal Disorder and Tumors". Biomedicines. 5 (4): 58. doi:10.3390/biomedicines5040058. PMC 5744082. PMID 28961226. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5744082" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5744082</a> <a href="#fnref:121" class="footnote-back-ref">↩</a></p></li> <li id="fn:122"><p>Barriuso J, Nagaraju R, Hurlstone A (March 2015). "Zebrafish: a new companion for translational research in oncology". Clinical Cancer Research. 21 (5): 969–75. doi:10.1158/1078-0432.CCR-14-2921. PMC 5034890. PMID 25573382. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5034890" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5034890</a> <a href="#fnref:122" class="footnote-back-ref">↩</a></p></li> <li id="fn:123"><p>Burket CT, Montgomery JE, Thummel R, Kassen SC, LaFave MC, Langenau DM, et al. (April 2008). "Generation and characterization of transgenic zebrafish lines using different ubiquitous promoters". Transgenic Research. 17 (2): 265–79. doi:10.1007/s11248-007-9152-5. PMC 3660017. PMID 17968670. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3660017" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3660017</a> <a href="#fnref:123" class="footnote-back-ref">↩</a></p></li> <li id="fn:124"><p>Du SJ, Gong Z, Fletcher GL, Shears MA, King MJ, Idler DR, et al. (1992). "Growth Enhancement in Transgenic Atlantic Salmon by the Use of an 'All Fish' Chimeric Growth Hormone Gene Construct". Nature Biotechnology. 10 (2): 176–81. doi:10.1038/nbt0292-176. PMID 1368229. S2CID 27048646. <a href="/wiki/Doi_(identifier)" target="_blank">/wiki/Doi_(identifier)</a> <a href="#fnref:124" class="footnote-back-ref">↩</a></p></li> <li id="fn:125"><p>Devlin RH, Biagi CA, Yesaki TY, Smailus DE, Byatt JC (February 2001). "Growth of domesticated transgenic fish". Nature. 409 (6822): 781–2. Bibcode:2001Natur.409..781D. doi:10.1038/35057314. PMID 11236982. S2CID 5293883. <a href="/wiki/Bibcode_(identifier)" target="_blank">/wiki/Bibcode_(identifier)</a> <a href="#fnref:125" class="footnote-back-ref">↩</a></p></li> <li id="fn:126"><p>Rahman MA, et al. (2001). "Growth and nutritional trials on transgenic Nile tilapia containing an exogenous fish growth hormone gene". Journal of Fish Biology. 59 (1): 62–78. Bibcode:2001JFBio..59...62R. doi:10.1111/j.1095-8649.2001.tb02338.x. <a href="/wiki/Bibcode_(identifier)" target="_blank">/wiki/Bibcode_(identifier)</a> <a href="#fnref:126" class="footnote-back-ref">↩</a></p></li> <li id="fn:127"><p>Pollack A (21 December 2012). "Engineered Fish Moves a Step Closer to Approval". The New York Times. <a href="https://www.nytimes.com/2012/12/22/business/gene-altered-fish-moves-closer-to-federal-approval.html" target="_blank">https://www.nytimes.com/2012/12/22/business/gene-altered-fish-moves-closer-to-federal-approval.html</a> <a href="#fnref:127" class="footnote-back-ref">↩</a></p></li> <li id="fn:128"><p>"FDA: Genetically engineered fish would not harm nature". USA Today. 2012. Retrieved November 28, 2015. <a href="https://www.usatoday.com/story/news/nation/2012/12/21/fda-salmon-nature/1784933/" target="_blank">https://www.usatoday.com/story/news/nation/2012/12/21/fda-salmon-nature/1784933/</a> <a href="#fnref:128" class="footnote-back-ref">↩</a></p></li> <li id="fn:129"><p>"FDA: Genetically engineered fish would not harm nature". USA Today. 2012. Retrieved November 28, 2015. <a href="https://www.usatoday.com/story/news/nation/2012/12/21/fda-salmon-nature/1784933/" target="_blank">https://www.usatoday.com/story/news/nation/2012/12/21/fda-salmon-nature/1784933/</a> <a href="#fnref:129" class="footnote-back-ref">↩</a></p></li> <li id="fn:130"><p>Firger, J. (2014). "Controversy swims around genetically modified fish". CBS News. Retrieved November 28, 2015. <a href="http://www.cbsnews.com/news/controversy-swims-around-genetically-modified-fish/" target="_blank">http://www.cbsnews.com/news/controversy-swims-around-genetically-modified-fish/</a> <a href="#fnref:130" class="footnote-back-ref">↩</a></p></li> <li id="fn:131"><p>Firger, J. (2014). "Controversy swims around genetically modified fish". CBS News. Retrieved November 28, 2015. <a href="http://www.cbsnews.com/news/controversy-swims-around-genetically-modified-fish/" target="_blank">http://www.cbsnews.com/news/controversy-swims-around-genetically-modified-fish/</a> <a href="#fnref:131" class="footnote-back-ref">↩</a></p></li> <li id="fn:132"><p>Environmental Assessment for AquAdvantage Salmon <a href="https://www.fda.gov/downloads/AdvisoryCommittees/CommitteesMeetingMaterials/VeterinaryMedicineAdvisoryCommittee/UCM224760.pdf" target="_blank">https://www.fda.gov/downloads/AdvisoryCommittees/CommitteesMeetingMaterials/VeterinaryMedicineAdvisoryCommittee/UCM224760.pdf</a> <a href="#fnref:132" class="footnote-back-ref">↩</a></p></li> <li id="fn:133"><p>Steenhuysen, J., Polansek, T. (November 19, 2015). "U.S. clears genetically modified salmon for human consumption". Reuters. Retrieved November 20, 2015. <a href="https://www.reuters.com/article/us-aquabounty-technologies-fda-idUSKCN0T826T20151120#yDLkHQMESrDJvbit.97" target="_blank">https://www.reuters.com/article/us-aquabounty-technologies-fda-idUSKCN0T826T20151120#yDLkHQMESrDJvbit.97</a> <a href="#fnref:133" class="footnote-back-ref">↩</a></p></li> <li id="fn:134"><p>"AquAdvantage Salmon". FDA. Retrieved 20 July 2018. <a href="https://www.fda.gov/animalveterinary/developmentapprovalprocess/geneticengineering/geneticallyengineeredanimals/ucm280853.htm" target="_blank">https://www.fda.gov/animalveterinary/developmentapprovalprocess/geneticengineering/geneticallyengineeredanimals/ucm280853.htm</a> <a href="#fnref:134" class="footnote-back-ref">↩</a></p></li> <li id="fn:135"><p>"FDA Has Determined That the AquAdvantage Salmon is as Safe to Eat as Non-GE Salmon". U.S. Food & Drug Administration. 19 November 2015. Retrieved 9 February 2018. <a href="https://www.fda.gov/ForConsumers/ConsumerUpdates/ucm472487.htm" target="_blank">https://www.fda.gov/ForConsumers/ConsumerUpdates/ucm472487.htm</a> <a href="#fnref:135" class="footnote-back-ref">↩</a></p></li> <li id="fn:136"><p>Steenhuysen, J., Polansek, T. (November 19, 2015). "U.S. clears genetically modified salmon for human consumption". Reuters. Retrieved November 20, 2015. <a href="https://www.reuters.com/article/us-aquabounty-technologies-fda-idUSKCN0T826T20151120#yDLkHQMESrDJvbit.97" target="_blank">https://www.reuters.com/article/us-aquabounty-technologies-fda-idUSKCN0T826T20151120#yDLkHQMESrDJvbit.97</a> <a href="#fnref:136" class="footnote-back-ref">↩</a></p></li> <li id="fn:137"><p>"FDA: Genetically engineered fish would not harm nature". USA Today. 2012. Retrieved November 28, 2015. <a href="https://www.usatoday.com/story/news/nation/2012/12/21/fda-salmon-nature/1784933/" target="_blank">https://www.usatoday.com/story/news/nation/2012/12/21/fda-salmon-nature/1784933/</a> <a href="#fnref:137" class="footnote-back-ref">↩</a></p></li> <li id="fn:138"><p>"FDA: Genetically engineered fish would not harm nature". USA Today. 2012. Retrieved November 28, 2015. <a href="https://www.usatoday.com/story/news/nation/2012/12/21/fda-salmon-nature/1784933/" target="_blank">https://www.usatoday.com/story/news/nation/2012/12/21/fda-salmon-nature/1784933/</a> <a href="#fnref:138" class="footnote-back-ref">↩</a></p></li> <li id="fn:139"><p>Connor S. (2012). "Ready to eat: the first GM fish for the dinner table". The Independent. Retrieved November 28, 2015. <a href="https://www.independent.co.uk/news/science/ready-to-eat-the-first-gm-fish-for-the-dinner-table-8430639.html" target="_blank">https://www.independent.co.uk/news/science/ready-to-eat-the-first-gm-fish-for-the-dinner-table-8430639.html</a> <a href="#fnref:139" class="footnote-back-ref">↩</a></p></li> <li id="fn:140"><p>"Online Education Kit: 1981–82: First Transgenic Mice and Fruit Flies". genome.gov. <a href="http://www.genome.gov/25520307" target="_blank">http://www.genome.gov/25520307</a> <a href="#fnref:140" class="footnote-back-ref">↩</a></p></li> <li id="fn:141"><p>Weasner BM, Zhu J, Kumar JP (2017). "FLPing Genes on and off in Drosophila". Site-Specific Recombinases. Methods in Molecular Biology. Vol. 1642. pp. 195–209. doi:10.1007/978-1-4939-7169-5_13. ISBN 978-1-4939-7167-1. PMC 5858584. PMID 28815502. <a href="978-1-4939-7167-1" target="_blank">978-1-4939-7167-1</a> <a href="#fnref:141" class="footnote-back-ref">↩</a></p></li> <li id="fn:142"><p>Jennings BH (2011-05-01). "Drosophila – a versatile model in biology & medicine". Materials Today. 14 (5): 190–195. doi:10.1016/S1369-7021(11)70113-4. <a href="https://doi.org/10.1016%2FS1369-7021%2811%2970113-4" target="_blank">https://doi.org/10.1016%2FS1369-7021%2811%2970113-4</a> <a href="#fnref:142" class="footnote-back-ref">↩</a></p></li> <li id="fn:143"><p>Ren X, Holsteens K, Li H, Sun J, Zhang Y, Liu LP, et al. (May 2017). "Genome editing in Drosophila melanogaster: from basic genome engineering to the multipurpose CRISPR-Cas9 system". Science China Life Sciences. 60 (5): 476–489. doi:10.1007/s11427-017-9029-9. PMID 28527116. S2CID 4341967. <a href="/wiki/Doi_(identifier)" target="_blank">/wiki/Doi_(identifier)</a> <a href="#fnref:143" class="footnote-back-ref">↩</a></p></li> <li id="fn:144"><p>Gallagher, James "GM mosquitoes offer malaria hope" BBC News, Health, 20 April 2011. Retrieved 22 April 2011 <a href="https://www.bbc.co.uk/news/health-13128327" target="_blank">https://www.bbc.co.uk/news/health-13128327</a> <a href="#fnref:144" class="footnote-back-ref">↩</a></p></li> <li id="fn:145"><p>Corby-Harris V, Drexler A, Watkins de Jong L, Antonova Y, Pakpour N, Ziegler R, et al. (July 2010). Vernick KD (ed.). "Activation of Akt signaling reduces the prevalence and intensity of malaria parasite infection and lifespan in Anopheles stephensi mosquitoes". PLOS Pathogens. 6 (7): e1001003. doi:10.1371/journal.ppat.1001003. PMC 2904800. PMID 20664791. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2904800" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2904800</a> <a href="#fnref:145" class="footnote-back-ref">↩</a></p></li> <li id="fn:146"><p>Windbichler N, Menichelli M, Papathanos PA, Thyme SB, Li H, Ulge UY, et al. (May 2011). "A synthetic homing endonuclease-based gene drive system in the human malaria mosquito". Nature. 473 (7346): 212–5. Bibcode:2011Natur.473..212W. doi:10.1038/nature09937. PMC 3093433. PMID 21508956. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3093433" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3093433</a> <a href="#fnref:146" class="footnote-back-ref">↩</a></p></li> <li id="fn:147"><p>Wise de Valdez MR, Nimmo D, Betz J, Gong HF, James AA, Alphey L, et al. (March 2011). "Genetic elimination of dengue vector mosquitoes". Proceedings of the National Academy of Sciences of the United States of America. 108 (12): 4772–5. Bibcode:2011PNAS..108.4772W. doi:10.1073/pnas.1019295108. PMC 3064365. PMID 21383140. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3064365" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3064365</a> <a href="#fnref:147" class="footnote-back-ref">↩</a></p></li> <li id="fn:148"><p>Knapton S (6 February 2016). "Releasing millions of GM mosquitoes 'could solve zika crisis'". The Telegraph. Retrieved 14 March 2016. <a href="https://www.telegraph.co.uk/news/worldnews/zika/12143563/Releasing-millions-of-GM-mosquitoes-could-solve-zika-crisis.html" target="_blank">https://www.telegraph.co.uk/news/worldnews/zika/12143563/Releasing-millions-of-GM-mosquitoes-could-solve-zika-crisis.html</a> <a href="#fnref:148" class="footnote-back-ref">↩</a></p></li> <li id="fn:149"><p>Harris AF, Nimmo D, McKemey AR, Kelly N, Scaife S, Donnelly CA, et al. (October 2011). "Field performance of engineered male mosquitoes". Nature Biotechnology. 29 (11): 1034–7. doi:10.1038/nbt.2019. PMID 22037376. S2CID 30862975. <a href="/wiki/Doi_(identifier)" target="_blank">/wiki/Doi_(identifier)</a> <a href="#fnref:149" class="footnote-back-ref">↩</a></p></li> <li id="fn:150"><p>Staff (March 2011) "Cayman demonstrates RIDL potential" Oxitec Newsletter, March 2011. Retrieved 20 September 2011 <a href="https://web.archive.org/web/20111110150441/http://www.oxitec.com/wp-content/uploads/2011/03/OXITEC-Newsletter-March-11-Final.pdf" target="_blank">https://web.archive.org/web/20111110150441/http://www.oxitec.com/wp-content/uploads/2011/03/OXITEC-Newsletter-March-11-Final.pdf</a> <a href="#fnref:150" class="footnote-back-ref">↩</a></p></li> <li id="fn:151"><p>Knapton S (6 February 2016). "Releasing millions of GM mosquitoes 'could solve zika crisis'". The Telegraph. Retrieved 14 March 2016. <a href="https://www.telegraph.co.uk/news/worldnews/zika/12143563/Releasing-millions-of-GM-mosquitoes-could-solve-zika-crisis.html" target="_blank">https://www.telegraph.co.uk/news/worldnews/zika/12143563/Releasing-millions-of-GM-mosquitoes-could-solve-zika-crisis.html</a> <a href="#fnref:151" class="footnote-back-ref">↩</a></p></li> <li id="fn:152"><p>Benedict MQ, Robinson AS (August 2003). "The first releases of transgenic mosquitoes: an argument for the sterile insect technique". Trends in Parasitology. 19 (8): 349–55. doi:10.1016/s1471-4922(03)00144-2. PMID 12901936. <a href="/wiki/Doi_(identifier)" target="_blank">/wiki/Doi_(identifier)</a> <a href="#fnref:152" class="footnote-back-ref">↩</a></p></li> <li id="fn:153"><p>Zhang S (2017-09-08). "Genetically Modified Moths Come to New York". The Atlantic. Retrieved 2018-12-23. <a href="https://www.theatlantic.com/science/archive/2017/09/genetically-modified-sterile-insects-take-flight/539040/" target="_blank">https://www.theatlantic.com/science/archive/2017/09/genetically-modified-sterile-insects-take-flight/539040/</a> <a href="#fnref:153" class="footnote-back-ref">↩</a></p></li> <li id="fn:154"><p>Scharping N (2017-05-10). "After Mosquitos, Moths Are the Next Target For Genetic Engineering". Discover Magazine. Archived from the original on 2019-11-11. Retrieved 2018-12-23. <a href="https://web.archive.org/web/20191111080853/http://blogs.discovermagazine.com/d-brief/2017/05/10/genetic-engineering-moths/" target="_blank">https://web.archive.org/web/20191111080853/http://blogs.discovermagazine.com/d-brief/2017/05/10/genetic-engineering-moths/</a> <a href="#fnref:154" class="footnote-back-ref">↩</a></p></li> <li id="fn:155"><p>Zhang S (2017-09-08). "Genetically Modified Moths Come to New York". The Atlantic. Retrieved 2018-12-23. <a href="https://www.theatlantic.com/science/archive/2017/09/genetically-modified-sterile-insects-take-flight/539040/" target="_blank">https://www.theatlantic.com/science/archive/2017/09/genetically-modified-sterile-insects-take-flight/539040/</a> <a href="#fnref:155" class="footnote-back-ref">↩</a></p></li> <li id="fn:156"><p>Reeves R, Phillipson M (January 2017). "Mass Releases of Genetically Modified Insects in Area-Wide Pest Control Programs and Their Impact on Organic Farmers". Sustainability. 9 (1): 59. Bibcode:2017Sust....9...59R. doi:10.3390/su9010059. <a href="https://doi.org/10.3390%2Fsu9010059" target="_blank">https://doi.org/10.3390%2Fsu9010059</a> <a href="#fnref:156" class="footnote-back-ref">↩</a></p></li> <li id="fn:157"><p>Simmons GS, McKemey AR, Morrison NI, O'Connell S, Tabashnik BE, Claus J, et al. (2011-09-13). "Field performance of a genetically engineered strain of pink bollworm". PLOS ONE. 6 (9): e24110. Bibcode:2011PLoSO...624110S. doi:10.1371/journal.pone.0024110. PMC 3172240. PMID 21931649. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3172240" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3172240</a> <a href="#fnref:157" class="footnote-back-ref">↩</a></p></li> <li id="fn:158"><p>Xu H, O'Brochta DA (July 2015). "Advanced technologies for genetically manipulating the silkworm Bombyx mori, a model Lepidopteran insect". Proceedings. Biological Sciences. 282 (1810): 20150487. doi:10.1098/rspb.2015.0487. PMC 4590473. PMID 26108630. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4590473" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4590473</a> <a href="#fnref:158" class="footnote-back-ref">↩</a></p></li> <li id="fn:159"><p>Tomita M (April 2011). "Transgenic silkworms that weave recombinant proteins into silk cocoons". Biotechnology Letters. 33 (4): 645–54. doi:10.1007/s10529-010-0498-z. PMID 21184136. S2CID 25310446. <a href="/wiki/Doi_(identifier)" target="_blank">/wiki/Doi_(identifier)</a> <a href="#fnref:159" class="footnote-back-ref">↩</a></p></li> <li id="fn:160"><p>Xu J, Dong Q, Yu Y, Niu B, Ji D, Li M, et al. (August 2018). "Bombyx mori". Proceedings of the National Academy of Sciences of the United States of America. 115 (35): 8757–8762. doi:10.1073/pnas.1806805115. PMC 6126722. PMID 30082397. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6126722" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6126722</a> <a href="#fnref:160" class="footnote-back-ref">↩</a></p></li> <li id="fn:161"><p>Le Page M. "GM worms make a super-silk completely unknown in nature". New Scientist. Retrieved 2018-12-23. <a href="https://www.newscientist.com/article/2165351-gm-worms-make-a-super-silk-completely-unknown-in-nature/" target="_blank">https://www.newscientist.com/article/2165351-gm-worms-make-a-super-silk-completely-unknown-in-nature/</a> <a href="#fnref:161" class="footnote-back-ref">↩</a></p></li> <li id="fn:162"><p>Scott, B.B., Lois, C. (2005). "Generation of tissue-specific transgenic birds with lentiviral vectors". Proc. Natl. Acad. Sci. U.S.A. 102 (45): 16443–16447. Bibcode:2005PNAS..10216443S. doi:10.1073/pnas.0508437102. PMC 1275601. PMID 16260725. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1275601" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1275601</a> <a href="#fnref:162" class="footnote-back-ref">↩</a></p></li> <li id="fn:163"><p>"Poultry scientists develop transgenic chicken to aid study of embryo development". projects.ncsu.edu. Retrieved 2018-12-23. <a href="https://projects.ncsu.edu/cals/agcomm/magazine/spring03/transgenic.htm" target="_blank">https://projects.ncsu.edu/cals/agcomm/magazine/spring03/transgenic.htm</a> <a href="#fnref:163" class="footnote-back-ref">↩</a></p></li> <li id="fn:164"><p>"Genetically modified chickens that don't transmit bird flu developed; Breakthrough could prevent future bird flu epidemics". ScienceDaily. Retrieved 2018-12-23. <a href="https://www.sciencedaily.com/releases/2011/01/110113141601.htm" target="_blank">https://www.sciencedaily.com/releases/2011/01/110113141601.htm</a> <a href="#fnref:164" class="footnote-back-ref">↩</a></p></li> <li id="fn:165"><p>Botelho JF, Smith-Paredes D, Soto-Acuña S, O'Connor J, Palma V, Vargas AO (March 2016). "Molecular development of fibular reduction in birds and its evolution from dinosaurs". Evolution; International Journal of Organic Evolution. 70 (3): 543–54. doi:10.1111/evo.12882. PMC 5069580. PMID 26888088. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5069580" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5069580</a> <a href="#fnref:165" class="footnote-back-ref">↩</a></p></li> <li id="fn:166"><p>Becker R (2015). "US government approves transgenic chicken". Nature News. doi:10.1038/nature.2015.18985. S2CID 181399746. <a href="http://www.nature.com/news/us-government-approves-transgenic-chicken-1.18985" target="_blank">http://www.nature.com/news/us-government-approves-transgenic-chicken-1.18985</a> <a href="#fnref:166" class="footnote-back-ref">↩</a></p></li> <li id="fn:167"><p>"GM chickens that don't transmit bird flu". The University of Edinburgh. Retrieved September 3, 2015. <a href="http://www.roslin.ed.ac.uk/public-interest/gm-chickens/" target="_blank">http://www.roslin.ed.ac.uk/public-interest/gm-chickens/</a> <a href="#fnref:167" class="footnote-back-ref">↩</a></p></li> <li id="fn:168"><p>Landers J (November 10, 2014). "Paleontologist Jack Horner is hard at work trying to turn a chicken into a dinosaur". The Washington Times. Retrieved January 19, 2015. <a href="https://www.washingtonpost.com/national/health-science/paleontologist-jack-horner-is-hard-at-work-trying-to-turn-a-chicken-into-a-dinosaur/2014/11/10/cb35e46e-4e59-11e4-babe-e91da079cb8a_story.html" target="_blank">https://www.washingtonpost.com/national/health-science/paleontologist-jack-horner-is-hard-at-work-trying-to-turn-a-chicken-into-a-dinosaur/2014/11/10/cb35e46e-4e59-11e4-babe-e91da079cb8a_story.html</a> <a href="#fnref:168" class="footnote-back-ref">↩</a></p></li> <li id="fn:169"><p>Horner JR, Gorman J (2009). How to build a dinosaur: extinction doesn't have to be forever. New York: Dutton. ISBN 978-0-525-95104-9. OCLC 233549535. <a href="978-0-525-95104-9" target="_blank">978-0-525-95104-9</a> <a href="#fnref:169" class="footnote-back-ref">↩</a></p></li> <li id="fn:170"><p>Reverse Engineering Birds' Beaks Into Dinosaur Bones by Carl Zimmer, NY Times, May 12, 2015 <a href="https://www.nytimes.com/2015/05/12/science/reverse-engineering-birds-beaks-into-dinosaur-bones.html" target="_blank">https://www.nytimes.com/2015/05/12/science/reverse-engineering-birds-beaks-into-dinosaur-bones.html</a> <a href="#fnref:170" class="footnote-back-ref">↩</a></p></li> <li id="fn:171"><p>Botelho JF, Smith-Paredes D, Soto-Acuña S, O'Connor J, Palma V, Vargas AO (March 2016). "Molecular development of fibular reduction in birds and its evolution from dinosaurs". Evolution; International Journal of Organic Evolution. 70 (3): 543–54. doi:10.1111/evo.12882. PMC 5069580. PMID 26888088. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5069580" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5069580</a> <a href="#fnref:171" class="footnote-back-ref">↩</a></p></li> <li id="fn:172"><p>Francisco Botelho J, Smith-Paredes D, Soto-Acuña S, Mpodozis J, Palma V, Vargas AO (May 2015). "Skeletal plasticity in response to embryonic muscular activity underlies the development and evolution of the perching digit of birds". Scientific Reports. 5: 9840. Bibcode:2015NatSR...5.9840F. doi:10.1038/srep09840. PMC 4431314. PMID 25974685. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4431314" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4431314</a> <a href="#fnref:172" class="footnote-back-ref">↩</a></p></li> <li id="fn:173"><p>"Glowing biomarker could simplify in ovo chick sexing". WATTPoultry.com. 2023-02-20. Retrieved 2023-06-29. <a href="https://www.wattagnet.com/egg/egg-production/article/15537481/glowing-biomarker-could-simplify-in-ovo-chick-sexing" target="_blank">https://www.wattagnet.com/egg/egg-production/article/15537481/glowing-biomarker-could-simplify-in-ovo-chick-sexing</a> <a href="#fnref:173" class="footnote-back-ref">↩</a></p></li> <li id="fn:174"><p>"Israeli startup breeds hens which lay eggs of female-only chicks". ctech. 2022-12-13. Retrieved 2023-06-29. <a href="https://www.calcalistech.com/ctechnews/article/bkc3iyioj" target="_blank">https://www.calcalistech.com/ctechnews/article/bkc3iyioj</a> <a href="#fnref:174" class="footnote-back-ref">↩</a></p></li> <li id="fn:175"><p>"In-Ovo Sexing Overview". Innovate Animal Ag. Retrieved 2023-06-29. <a href="https://www.innovateanimalag.org/egg-sexing" target="_blank">https://www.innovateanimalag.org/egg-sexing</a> <a href="#fnref:175" class="footnote-back-ref">↩</a></p></li> <li id="fn:176"><p>Chesneau, A., Sachs, L. M., Chai, N., Chen, Y., Du Pasquier, L., Loeber, J., et al. (2008). "Transgenesis procedures in Xenopus". Biology of the Cell. 100 (9): 503–529. doi:10.1042/BC20070148. ISSN 1768-322X. PMC 2967756. PMID 18699776. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2967756" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2967756</a> <a href="#fnref:176" class="footnote-back-ref">↩</a></p></li> <li id="fn:177"><p>Sobkow, L., Epperlein, H.-H., Herklotz, S., Straube, W. L., Tanaka, E. M. (February 2006). "A germline GFP transgenic axolotl and its use to track cell fate: Dual origin of the fin mesenchyme during development and the fate of blood cells during regeneration". Developmental Biology. 290 (2): 386–397. doi:10.1016/j.ydbio.2005.11.037. ISSN 0012-1606. PMID 16387293. <a href="https://doi.org/10.1016%2Fj.ydbio.2005.11.037" target="_blank">https://doi.org/10.1016%2Fj.ydbio.2005.11.037</a> <a href="#fnref:177" class="footnote-back-ref">↩</a></p></li> <li id="fn:178"><p>Echeverri, K., Fei, J., Tanaka, E. M. (2022). "The Axolotl's journey to the modern molecular era". Emerging Model Systems in Developmental Biology. Current Topics in Developmental Biology. Vol. 147. Elsevier. pp. 631–658. doi:10.1016/bs.ctdb.2021.12.010. ISBN 978-0-12-820154-1. PMC 10029325. PMID 35337465. <a href="978-0-12-820154-1" target="_blank">978-0-12-820154-1</a> <a href="#fnref:178" class="footnote-back-ref">↩</a></p></li> <li id="fn:179"><p>Fini JB, Le Mevel S, Turque N, Palmier K, Zalko D, Cravedi JP, et al. (August 2007). "An in vivo multiwell-based fluorescent screen for monitoring vertebrate thyroid hormone disruption". Environmental Science & Technology. 41 (16): 5908–14. Bibcode:2007EnST...41.5908F. doi:10.1021/es0704129. PMID 17874805. <a href="https://figshare.com/articles/An_In_Vivo_Multiwell_Based_Fluorescent_Screen_for_Monitoring_Vertebrate_Thyroid_Hormone_Disruption/2991250" target="_blank">https://figshare.com/articles/An_In_Vivo_Multiwell_Based_Fluorescent_Screen_for_Monitoring_Vertebrate_Thyroid_Hormone_Disruption/2991250</a> <a href="#fnref:179" class="footnote-back-ref">↩</a></p></li> <li id="fn:180"><p>"Removing Threat from Invasive Species with Genetic Engineering?". Science in the News. 2014-07-28. Retrieved 2018-12-23. <a href="http://sitn.hms.harvard.edu/flash/2014/removing-threat-from-invasive-species-with-genetic-engineering/" target="_blank">http://sitn.hms.harvard.edu/flash/2014/removing-threat-from-invasive-species-with-genetic-engineering/</a> <a href="#fnref:180" class="footnote-back-ref">↩</a></p></li> <li id="fn:181"><p>"Cane toads to get the Crispr treatment". Radio National. 2017-11-17. Retrieved 2018-12-23. <a href="https://www.abc.net.au/radionational/programs/scienceshow/cane-toads-to-get-the-crispr-treatment/9161942" target="_blank">https://www.abc.net.au/radionational/programs/scienceshow/cane-toads-to-get-the-crispr-treatment/9161942</a> <a href="#fnref:181" class="footnote-back-ref">↩</a></p></li> <li id="fn:182"><p>Horb, M., Wlizla, M., Abu-Daya, A., McNamara, S., Gajdasik, D., Igawa, T., et al. (2019). "Xenopus Resources: Transgenic, Inbred and Mutant Animals, Training Opportunities, and Web-Based Support". Frontiers in Physiology. 10: 387. doi:10.3389/fphys.2019.00387. ISSN 1664-042X. PMC 6497014. PMID 31073289. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6497014" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6497014</a> <a href="#fnref:182" class="footnote-back-ref">↩</a></p></li> <li id="fn:183"><p>Suzuki, N., Ochi, H. (2020). "Regeneration enhancers: A clue to reactivation of developmental genes". Development, Growth & Differentiation. 62 (5): 343–354. doi:10.1111/dgd.12654. ISSN 1440-169X. PMC 7383998. PMID 32096563. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7383998" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7383998</a> <a href="#fnref:183" class="footnote-back-ref">↩</a></p></li> <li id="fn:184"><p>Horb, M., Wlizla, M., Abu-Daya, A., McNamara, S., Gajdasik, D., Igawa, T., et al. (2019). "Xenopus Resources: Transgenic, Inbred and Mutant Animals, Training Opportunities, and Web-Based Support". Frontiers in Physiology. 10: 387. doi:10.3389/fphys.2019.00387. ISSN 1664-042X. PMC 6497014. PMID 31073289. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6497014" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6497014</a> <a href="#fnref:184" class="footnote-back-ref">↩</a></p></li> <li id="fn:185"><p>Gesslbauer, B., Radtke, C. (November 2018). "The Regenerative Capability of the Urodele Amphibians and Its Potential for Plastic Surgery". Annals of Plastic Surgery. 81 (5): 511–515. doi:10.1097/SAP.0000000000001619. ISSN 1536-3708. PMID 30247194. S2CID 52350332. <a href="/wiki/Doi_(identifier)" target="_blank">/wiki/Doi_(identifier)</a> <a href="#fnref:185" class="footnote-back-ref">↩</a></p></li> <li id="fn:186"><p>Pollet N, Mazabraud A (2006). "Insights from Xenopus Genomes". In Volff JN (ed.). Vertebrate genomes. Genome Dynamics (in German). Vol. 2. Basel, Switzerland: Karger. pp. 138–153. doi:10.1159/000095101. ISBN 978-3-8055-8151-6. OCLC 69391396. PMID 18753776. <a href="978-3-8055-8151-6" target="_blank">978-3-8055-8151-6</a> <a href="#fnref:186" class="footnote-back-ref">↩</a></p></li> <li id="fn:187"><p>Sobkow, L., Epperlein, H.-H., Herklotz, S., Straube, W. L., Tanaka, E. M. (February 2006). "A germline GFP transgenic axolotl and its use to track cell fate: Dual origin of the fin mesenchyme during development and the fate of blood cells during regeneration". Developmental Biology. 290 (2): 386–397. doi:10.1016/j.ydbio.2005.11.037. ISSN 0012-1606. PMID 16387293. <a href="https://doi.org/10.1016%2Fj.ydbio.2005.11.037" target="_blank">https://doi.org/10.1016%2Fj.ydbio.2005.11.037</a> <a href="#fnref:187" class="footnote-back-ref">↩</a></p></li> <li id="fn:188"><p>Pollet N, Mazabraud A (2006). "Insights from Xenopus Genomes". In Volff JN (ed.). Vertebrate genomes. Genome Dynamics (in German). Vol. 2. Basel, Switzerland: Karger. pp. 138–153. doi:10.1159/000095101. ISBN 978-3-8055-8151-6. OCLC 69391396. PMID 18753776. <a href="978-3-8055-8151-6" target="_blank">978-3-8055-8151-6</a> <a href="#fnref:188" class="footnote-back-ref">↩</a></p></li> <li id="fn:189"><p>Beck, C. W., Slack, J. M. (19 September 2001). "An amphibian with ambition: a new role for Xenopus in the 21st century". Genome Biology. 2 (10): reviews1029.1. doi:10.1186/gb-2001-2-10-reviews1029. ISSN 1474-760X. PMC 138973. PMID 11597339. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC138973" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC138973</a> <a href="#fnref:189" class="footnote-back-ref">↩</a></p></li> <li id="fn:190"><p>Sobkow, L., Epperlein, H.-H., Herklotz, S., Straube, W. L., Tanaka, E. M. (February 2006). "A germline GFP transgenic axolotl and its use to track cell fate: Dual origin of the fin mesenchyme during development and the fate of blood cells during regeneration". Developmental Biology. 290 (2): 386–397. doi:10.1016/j.ydbio.2005.11.037. ISSN 0012-1606. PMID 16387293. <a href="https://doi.org/10.1016%2Fj.ydbio.2005.11.037" target="_blank">https://doi.org/10.1016%2Fj.ydbio.2005.11.037</a> <a href="#fnref:190" class="footnote-back-ref">↩</a></p></li> <li id="fn:191"><p>Tilley, L., Papadopoulos, S., Pende, M., Fei, J., Murawala, P. (13 May 2021). "The use of transgenics in the laboratory axolotl". Developmental Dynamics. 251 (6): 942–956. doi:10.1002/dvdy.357. eISSN 1097-0177. ISSN 1058-8388. PMC 8568732. PMID 33949035. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8568732" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8568732</a> <a href="#fnref:191" class="footnote-back-ref">↩</a></p></li> <li id="fn:192"><p>Tilley, L., Papadopoulos, S., Pende, M., Fei, J., Murawala, P. (13 May 2021). "The use of transgenics in the laboratory axolotl". Developmental Dynamics. 251 (6): 942–956. doi:10.1002/dvdy.357. eISSN 1097-0177. ISSN 1058-8388. PMC 8568732. PMID 33949035. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8568732" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8568732</a> <a href="#fnref:192" class="footnote-back-ref">↩</a></p></li> <li id="fn:193"><p>Echeverri, K., Fei, J., Tanaka, E. M. (2022). "The Axolotl's journey to the modern molecular era". Emerging Model Systems in Developmental Biology. Current Topics in Developmental Biology. Vol. 147. Elsevier. pp. 631–658. doi:10.1016/bs.ctdb.2021.12.010. ISBN 978-0-12-820154-1. PMC 10029325. PMID 35337465. <a href="978-0-12-820154-1" target="_blank">978-0-12-820154-1</a> <a href="#fnref:193" class="footnote-back-ref">↩</a></p></li> <li id="fn:194"><p>Tilley, L., Papadopoulos, S., Pende, M., Fei, J., Murawala, P. (13 May 2021). "The use of transgenics in the laboratory axolotl". Developmental Dynamics. 251 (6): 942–956. doi:10.1002/dvdy.357. eISSN 1097-0177. ISSN 1058-8388. PMC 8568732. PMID 33949035. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8568732" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8568732</a> <a href="#fnref:194" class="footnote-back-ref">↩</a></p></li> <li id="fn:195"><p>Tilley, L., Papadopoulos, S., Pende, M., Fei, J., Murawala, P. (13 May 2021). "The use of transgenics in the laboratory axolotl". Developmental Dynamics. 251 (6): 942–956. doi:10.1002/dvdy.357. eISSN 1097-0177. ISSN 1058-8388. PMC 8568732. PMID 33949035. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8568732" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8568732</a> <a href="#fnref:195" class="footnote-back-ref">↩</a></p></li> <li id="fn:196"><p>Steinhoff, G., ed. (2016). Regenerative Medicine - from Protocol to Patient. Springer International Publishing. doi:10.1007/978-3-319-27583-3. ISBN 978-3-319-27581-9. S2CID 27313520. <a href="978-3-319-27581-9" target="_blank">978-3-319-27581-9</a> <a href="#fnref:196" class="footnote-back-ref">↩</a></p></li> <li id="fn:197"><p>Woodcock, M. R., Vaughn-Wolfe, J., Elias, A., Kump, D. K., Kendall, K. D., Timoshevskaya, N., et al. (31 January 2017). "Identification of Mutant Genes and Introgressed Tiger Salamander DNA in the Laboratory Axolotl, Ambystoma mexicanum". Scientific Reports. 7 (1). Nature Publishing Group: 6. Bibcode:2017NatSR...7....6W. doi:10.1038/s41598-017-00059-1. ISSN 2045-2322. PMC 5428337. PMID 28127056. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5428337" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5428337</a> <a href="#fnref:197" class="footnote-back-ref">↩</a></p></li> <li id="fn:198"><p>Echeverri, K., Fei, J., Tanaka, E. M. (2022). "The Axolotl's journey to the modern molecular era". Emerging Model Systems in Developmental Biology. Current Topics in Developmental Biology. Vol. 147. Elsevier. pp. 631–658. doi:10.1016/bs.ctdb.2021.12.010. ISBN 978-0-12-820154-1. PMC 10029325. PMID 35337465. <a href="978-0-12-820154-1" target="_blank">978-0-12-820154-1</a> <a href="#fnref:198" class="footnote-back-ref">↩</a></p></li> <li id="fn:199"><p>Echeverri, K., Clarke, J. D. W., Tanaka, E. M. (August 2001). "In Vivo Imaging Indicates Muscle Fiber Dedifferentiation Is a Major Contributor to the Regenerating Tail Blastema". Developmental Biology. 236 (1): 151–164. doi:10.1006/dbio.2001.0312. ISSN 0012-1606. PMID 11456451. <a href="https://doi.org/10.1006%2Fdbio.2001.0312" target="_blank">https://doi.org/10.1006%2Fdbio.2001.0312</a> <a href="#fnref:199" class="footnote-back-ref">↩</a></p></li> <li id="fn:200"><p>Tilley, L., Papadopoulos, S., Pende, M., Fei, J., Murawala, P. (13 May 2021). "The use of transgenics in the laboratory axolotl". Developmental Dynamics. 251 (6): 942–956. doi:10.1002/dvdy.357. eISSN 1097-0177. ISSN 1058-8388. PMC 8568732. PMID 33949035. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8568732" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8568732</a> <a href="#fnref:200" class="footnote-back-ref">↩</a></p></li> <li id="fn:201"><p>Nowoshilow, S., Tanaka, E. M. (September 2020). "Introducing www.axolotl-omics.org – an integrated -omics data portal for the axolotl research community". Experimental Cell Research. 394 (1): 112143. doi:10.1016/j.yexcr.2020.112143. ISSN 0014-4827. PMID 32540400. S2CID 219704317. <a href="https://doi.org/10.1016%2Fj.yexcr.2020.112143" target="_blank">https://doi.org/10.1016%2Fj.yexcr.2020.112143</a> <a href="#fnref:201" class="footnote-back-ref">↩</a></p></li> <li id="fn:202"><p>Schloissnig, S., Kawaguchi, A., Nowoshilow, S., Falcon, F., Otsuki, L., Tardivo, P., et al. (13 April 2021). "The giant axolotl genome uncovers the evolution, scaling, and transcriptional control of complex gene loci". Proceedings of the National Academy of Sciences. 118 (15): e2017176118. Bibcode:2021PNAS..11817176S. doi:10.1073/pnas.2017176118. ISSN 1091-6490. PMC 8053990. PMID 33827918. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8053990" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8053990</a> <a href="#fnref:202" class="footnote-back-ref">↩</a></p></li> <li id="fn:203"><p>"History of research on C. elegans and other free-living nematodes as model organisms". www.wormbook.org. Retrieved 2018-12-24. <a href="http://www.wormbook.org/chapters/www_nematodeshistory/nematodeshistory.html" target="_blank">http://www.wormbook.org/chapters/www_nematodeshistory/nematodeshistory.html</a> <a href="#fnref:203" class="footnote-back-ref">↩</a></p></li> <li id="fn:204"><p>Hopkin M (2006-10-02). "RNAi scoops medical Nobel". News@nature. doi:10.1038/news061002-2. ISSN 1744-7933. S2CID 85168270. <a href="/wiki/Doi_(identifier)" target="_blank">/wiki/Doi_(identifier)</a> <a href="#fnref:204" class="footnote-back-ref">↩</a></p></li> <li id="fn:205"><p>Conte D, MacNeil LT, Walhout AJ, Mello CC (January 2015). "RNA Interference in Caenorhabditis elegans". Current Protocols in Molecular Biology. 109: 26.3.1–26.3.30. doi:10.1002/0471142727.mb2603s109. ISBN 978-0-471-14272-0. PMC 5396541. PMID 25559107. <a href="978-0-471-14272-0" target="_blank">978-0-471-14272-0</a> <a href="#fnref:205" class="footnote-back-ref">↩</a></p></li> <li id="fn:206"><p>Praitis V, Maduro MF (2011). "Transgenesis in C. elegans". Caenorhabditis elegans: Molecular Genetics and Development. Methods in Cell Biology. Vol. 106. pp. 161–85. doi:10.1016/B978-0-12-544172-8.00006-2. ISBN 978-0-12-544172-8. PMID 22118277. <a href="978-0-12-544172-8" target="_blank">978-0-12-544172-8</a> <a href="#fnref:206" class="footnote-back-ref">↩</a></p></li> <li id="fn:207"><p>Praitis V, Maduro MF (2011). "Transgenesis in C. elegans". Caenorhabditis elegans: Molecular Genetics and Development. Methods in Cell Biology. Vol. 106. pp. 161–85. doi:10.1016/B978-0-12-544172-8.00006-2. ISBN 978-0-12-544172-8. PMID 22118277. <a href="978-0-12-544172-8" target="_blank">978-0-12-544172-8</a> <a href="#fnref:207" class="footnote-back-ref">↩</a></p></li> <li id="fn:208"><p>Diogo J, Bratanich A (November 2014). "The nematode Caenorhabditis elegans as a model to study viruses". Archives of Virology. 159 (11): 2843–51. doi:10.1007/s00705-014-2168-2. PMID 25000902. S2CID 18865352. <a href="https://doi.org/10.1007%2Fs00705-014-2168-2" target="_blank">https://doi.org/10.1007%2Fs00705-014-2168-2</a> <a href="#fnref:208" class="footnote-back-ref">↩</a></p></li> <li id="fn:209"><p>Tejeda-Benitez L, Olivero-Verbel J (2016). "Caenorhabditis elegans, a Biological Model for Research in Toxicology". Reviews of Environmental Contamination and Toxicology Volume 237. Vol. 237. pp. 1–35. doi:10.1007/978-3-319-23573-8_1. ISBN 978-3-319-23572-1. PMID 26613986. <a href="978-3-319-23572-1" target="_blank">978-3-319-23572-1</a> <a href="#fnref:209" class="footnote-back-ref">↩</a></p></li> <li id="fn:210"><p>Schmidt J, Schmidt T (2018). "Animal Models of Machado-Joseph Disease". Polyglutamine Disorders. Advances in Experimental Medicine and Biology. Vol. 1049. pp. 289–308. doi:10.1007/978-3-319-71779-1_15. ISBN 978-3-319-71778-4. PMID 29427110. <a href="978-3-319-71778-4" target="_blank">978-3-319-71778-4</a> <a href="#fnref:210" class="footnote-back-ref">↩</a></p></li> <li id="fn:211"><p>Griffin EF, Caldwell KA, Caldwell GA (December 2017). "Genetic and Pharmacological Discovery for Alzheimer's Disease Using Caenorhabditis elegans". ACS Chemical Neuroscience. 8 (12): 2596–2606. doi:10.1021/acschemneuro.7b00361. PMID 29022701. <a href="/wiki/Doi_(identifier)" target="_blank">/wiki/Doi_(identifier)</a> <a href="#fnref:211" class="footnote-back-ref">↩</a></p></li> <li id="fn:212"><p>Daniells C, Mutwakil MH, Power RS, David HE, De Pomerai DI (2002). "Transgenic Nematodes as Biosensors of Environmental Stress". Biotechnology for the Environment: Strategy and Fundamentals. Focus on Biotechnology. Vol. 3A. Springer, Dordrecht. pp. 221–236. doi:10.1007/978-94-010-0357-5_15. ISBN 978-94-010-3907-9. <a href="978-94-010-3907-9" target="_blank">978-94-010-3907-9</a> <a href="#fnref:212" class="footnote-back-ref">↩</a></p></li> <li id="fn:213"><p>"More valuable than gold, but not for long: genetically-modified sea cucumbers headed to China's dinner tables". South China Morning Post. 2015-08-05. Retrieved 2018-12-23. <a href="https://www.scmp.com/tech/science-research/article/1846481/more-valuable-gold-not-long-genetically-modified-sea-cucumbers" target="_blank">https://www.scmp.com/tech/science-research/article/1846481/more-valuable-gold-not-long-genetically-modified-sea-cucumbers</a> <a href="#fnref:213" class="footnote-back-ref">↩</a></p></li> <li id="fn:214"><p>Zeng A, Li H, Guo L, Gao X, McKinney S, Wang Y, et al. (June 2018). "+ Neoblasts Are Adult Pluripotent Stem Cells Underlying Planaria Regeneration". Cell. 173 (7): 1593–1608.e20. doi:10.1016/j.cell.2018.05.006. PMC 9359418. PMID 29906446. S2CID 49238332. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9359418" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9359418</a> <a href="#fnref:214" class="footnote-back-ref">↩</a></p></li> <li id="fn:215"><p>"One special cell can revive a flatworm on the brink of death". Nature. 558 (7710): 346–347. 14 June 2018. Bibcode:2018Natur.558S.346.. doi:10.1038/d41586-018-05440-2. S2CID 49296244. <a href="http://www.nature.com/articles/d41586-018-05440-2" target="_blank">http://www.nature.com/articles/d41586-018-05440-2</a> <a href="#fnref:215" class="footnote-back-ref">↩</a></p></li> <li id="fn:216"><p>Wudarski J, Simanov D, Ustyantsev K, de Mulder K, Grelling M, Grudniewska M, et al. (December 2017). "Efficient transgenesis and annotated genome sequence of the regenerative flatworm model Macrostomum lignano". Nature Communications. 8 (1): 2120. Bibcode:2017NatCo...8.2120W. doi:10.1038/s41467-017-02214-8. PMC 5730564. PMID 29242515. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5730564" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5730564</a> <a href="#fnref:216" class="footnote-back-ref">↩</a></p></li> <li id="fn:217"><p>Zantke J, Bannister S, Rajan VB, Raible F, Tessmar-Raible K (May 2014). "Genetic and genomic tools for the marine annelid Platynereis dumerilii". Genetics. 197 (1): 19–31. doi:10.1534/genetics.112.148254. PMC 4012478. PMID 24807110. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4012478" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4012478</a> <a href="#fnref:217" class="footnote-back-ref">↩</a></p></li> <li id="fn:218"><p>Wittlieb J, Khalturin K, Lohmann JU, Anton-Erxleben F, Bosch TC (April 2006). "Transgenic Hydra allow in vivo tracking of individual stem cells during morphogenesis". Proceedings of the National Academy of Sciences of the United States of America. 103 (16): 6208–11. Bibcode:2006PNAS..103.6208W. doi:10.1073/pnas.0510163103. PMC 1458856. PMID 16556723. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1458856" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1458856</a> <a href="#fnref:218" class="footnote-back-ref">↩</a></p></li> <li id="fn:219"><p>Perry KJ, Henry JQ (February 2015). "CRISPR/Cas9-mediated genome modification in the mollusc, Crepidula fornicata". Genesis. 53 (2): 237–44. doi:10.1002/dvg.22843. PMID 25529990. S2CID 36057310. <a href="/wiki/Doi_(identifier)" target="_blank">/wiki/Doi_(identifier)</a> <a href="#fnref:219" class="footnote-back-ref">↩</a></p></li> <li id="fn:220"><p>Nomura T, Yamashita W, Gotoh H, Ono K (2015-02-24). "Genetic manipulation of reptilian embryos: toward an understanding of cortical development and evolution". Frontiers in Neuroscience. 9: 45. doi:10.3389/fnins.2015.00045. PMC 4338674. PMID 25759636. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4338674" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4338674</a> <a href="#fnref:220" class="footnote-back-ref">↩</a></p></li> <li id="fn:221"><p>Rasmussen RS, Morrissey MT (2007). "Biotechnology in Aquaculture: Transgenics and Polyploidy". Comprehensive Reviews in Food Science and Food Safety. 6 (1): 2–16. doi:10.1111/j.1541-4337.2007.00013.x. <a href="/wiki/Doi_(identifier)" target="_blank">/wiki/Doi_(identifier)</a> <a href="#fnref:221" class="footnote-back-ref">↩</a></p></li> <li id="fn:222"><p>Ebert MS, Sharp PA (November 2010). "MicroRNA sponges: progress and possibilities". RNA. 16 (11): 2043–50. doi:10.1261/rna.2414110. PMC 2957044. PMID 20855538. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2957044" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2957044</a> <a href="#fnref:222" class="footnote-back-ref">↩</a></p></li> <li id="fn:223"><p>Frewer L, Kleter G, Brennan M, Coles D, Fischer A, Houdebine L, et al. (June 2013). "Genetically modified animals from life-science, socio-economic and ethical perspectives: examining issues in an EU policy context". New Biotechnology. 30 (5): 447–460. doi:10.1016/j.nbt.2013.03.010. PMID 23567982. <a href="https://linkinghub.elsevier.com/retrieve/pii/S1871678413000435" target="_blank">https://linkinghub.elsevier.com/retrieve/pii/S1871678413000435</a> <a href="#fnref:223" class="footnote-back-ref">↩</a></p></li> <li id="fn:224"><p>Eriksson S, Jonas E, Rydhmer L, Röcklinsberg H (January 2018). "Invited review: Breeding and ethical perspectives on genetically modified and genome edited cattle". Journal of Dairy Science. 101 (1): 1–17. doi:10.3168/jds.2017-12962. PMID 29102147. <a href="https://doi.org/10.3168%2Fjds.2017-12962" target="_blank">https://doi.org/10.3168%2Fjds.2017-12962</a> <a href="#fnref:224" class="footnote-back-ref">↩</a></p></li> <li id="fn:225"><p>Kiani AK, Pheby D, Henehan G, Brown R, Sieving P, Sykora P, et al. (2022-10-17). "Ethical considerations regarding animal experimentation". Journal of Preventive Medicine and Hygiene. 63 (2S3): E255 – E266. doi:10.15167/2421-4248/JPMH2022.63.2S3.2768. PMC 9710398. PMID 36479489. <a href="https://www.jpmh.org/index.php/jpmh/article/view/2768" target="_blank">https://www.jpmh.org/index.php/jpmh/article/view/2768</a> <a href="#fnref:225" class="footnote-back-ref">↩</a></p></li> </ol>