1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide

1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC, EDAC or EDCI) is a water-soluble <a href="/facts/Carbodiimide/e02KT0rw">carbodiimide</a> usually handled as the <a href="/facts/Hydrochloride/7Sdqua4a">hydrochloride</a>. 
It is typically employed in the 4.0-6.0 <a href="/facts/PH/RnSv8D7y">pH</a> range. It is generally used as a carboxyl activating agent for the coupling of primary amines to yield amide bonds. While other carbodiimides like <a href="/facts/N,N%27-Dicyclohexylcarbodiimide/AbjRRd61">dicyclohexylcarbodiimide</a> (DCC) or <a href="/facts/N,N%27-Diisopropylcarbodiimide/osPrYxJM">diisopropylcarbodiimide</a> (DIC) are also employed for this purpose, EDC has the advantage that the urea byproduct formed (often challenging to remove in the case of DCC or DIC) can be washed away from the amide product using dilute acid. Additionally, EDC can also be used to activate <a href="/facts/Phosphate/FDGVCxUG">phosphate</a> groups in order to form phosphomonoesters and phosphodiesters. Common uses for this carbodiimide include <a href="/facts/Peptide_synthesis/Upd4aqKa">peptide synthesis</a>, <a href="/facts/Protein_crosslinking/zeQ9hEAA">protein crosslinking</a> to <a href="/facts/Nucleic_acid/2tTgcI0v">nucleic acids</a>, but also in the preparation of <a href="/facts/Immunoconjugate/9bIKtjfk">immunoconjugates</a>. EDC is often used in combination with <a href="/facts/N-Hydroxysuccinimide/IJFewttf">N-hydroxysuccinimide</a> (NHS) for the immobilisation of large <a href="/facts/Biomolecules/DGvFzN7R">biomolecules</a>. Recent work has also used EDC to <a href="/facts/Nucleic_acid_structure_determination/u2HmxFU3">assess the structure state</a> of uracil nucleobases in RNA.

1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide open-in-new

1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide