The enzyme cutinase (systematic name: cutin hydrolase, EC 3.1.1.74) is a member of the hydrolase family. It catalyzes the following reaction:
R 1 COOR 2 + H 2 O ⟶ R 1 COOH + R 2 OH {\displaystyle {\ce {R1COOR2 + H2O -> R1COOH + R2OH}}}
In biological systems, the reactant carboxylic ester is a constituent of the cutin polymer, and the hydrolysis of cutin results in the formation of alcohol and carboxylic acid monomer products.