Cycling probe technology

<p>Cycling probe technology (CPT) is a <a href="/facts/Molecular_biology/YgqU35ub">molecular biological</a> technique for detecting specific <a href="/facts/DNA/nB89Iauz">DNA</a> sequences. CPT operates under <a href="/facts/Isothermal/6mkdESuS">isothermal</a> conditions.  In some applications, CPT offers an alternative to <a href="/facts/Polymerase_chain_reaction/G0jWzJy1">PCR</a>. However, unlike PCR, CPT does not generate multiple copies of the target DNA itself, and the amplification of the signal is linear, in contrast to the exponential amplification of the target DNA in PCR. CPT uses a sequence specific chimeric probe which hybridizes to a complementary target DNA sequence and becomes a substrate for <a href="/facts/RNase_H/UKaLv8fh">RNase H</a>. Cleavage occurs at the RNA internucleotide linkages and results in dissociation of the probe from the target, thereby making it available for the next probe molecule. <a href="/facts/Microfluidic_chip/5ABHeIdC">Integrated electrokinetic systems</a> have been developed for use in CPT.
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Cycling probe technology open-in-new

Cycling probe technology