Restriction digest

A restriction digest is a procedure used in <a href="/facts/Molecular_biology/YgqU35ub">molecular biology</a> to prepare <a href="/facts/Deoxyribonucleic_acid/nB89Iauz">DNA</a> for analysis or other processing. It is sometimes termed DNA fragmentation, though this term is used for other procedures as well. In a restriction digest, DNA molecules are cleaved at specific restriction sites of 4-12 nucleotides in length by use of restriction enzymes which recognize these sequences. 
The resulting digested DNA is very often selectively amplified using <a href="/facts/Polymerase_chain_reaction/G0jWzJy1">polymerase chain reaction</a> (PCR), making it more suitable for analytical techniques such as <a href="/facts/Agarose_gel_electrophoresis/JxY5UUXv">agarose gel electrophoresis</a>, and <a href="/facts/Chromatography/2FrvPjNV">chromatography</a>. It is used in <a href="/facts/Genetic_fingerprinting/NhYPDDH4">genetic fingerprinting</a>, plasmid <a href="/facts/Subcloning/7aJ2uUc6">subcloning</a>, and <a href="/facts/Restriction_fragment_length_polymorphism/tJutAmFv">RFLP analysis</a>.

Restriction digest open-in-new

Restriction digest