Massive parallel sequencing

<p>Massive parallel sequencing or massively parallel sequencing is any of several high-throughput approaches to <a href="/facts/DNA_sequencing/hsPsNCQW">DNA sequencing</a> using the concept of massively parallel processing; it is also called next-generation sequencing (NGS) or second-generation sequencing. Some of these technologies emerged between 1993 and 1998  and have been commercially available since 2005. These technologies use miniaturized and parallelized platforms for sequencing of 1 million to 43 billion short reads (50 to 400 bases each) per instrument run.
</p><p>Many NGS platforms differ in engineering configurations and sequencing chemistry. They share the technical paradigm of massive parallel sequencing via spatially separated, clonally amplified <a href="/facts/DNA/nB89Iauz">DNA</a> templates or single DNA molecules in a <a href="/facts/Flow_cytometry/M2IvPdL8">flow cell</a>. This design is very different from that of <a href="/facts/Sanger_sequencing/PzqMpaaM">Sanger sequencing</a>—also known as capillary sequencing or first-generation sequencing—which is based on <a href="/facts/Electrophoretic/B0aLKHTt">electrophoretic</a> separation of chain-termination products produced in individual sequencing reactions. This methodology allows sequencing to be completed on a larger scale.
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Massive parallel sequencing open-in-new

Massive parallel sequencing